LOCUS       sequence-244836-        2710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus left homology arm; type 1 part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I2L
FEATURES             Location/Qualifiers
     source          1..2710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1073..1107)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1178..1837)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1752..1771
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2019..2607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2508..2527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcac cctttcaggc gcgccaccga atattaaata atcttttttt atttcttatg
       61 tacgcctgct ttgtatgata caaaatcatc gtatcgtatt cggcttcatg ttatccgtcg
      121 aatttagatt accttggcta aggggccaag gtggatgatt cgtatgagta tcacaattta
      181 caaattacaa gttttgcaaa gagtatgtaa actgtaaaaa agaaaacatt gacaaatata
      241 cctgaaaaga gtccattttc tacatttgag aaaaaaagga ataaagtgat tctcctcatc
      301 gctcataaaa aaatactatt catgagcaaa attgagaaaa atggcaatca cattcaatac
      361 tagttagata gccatatcta aactgctgta cgtggtttaa tactagcacc aacatcattt
      421 gaataatggc gaagaaacca atttcgaaac attcccgtgc ggcaagaaga tcagaagcag
      481 ccgaaccgga ggctaaggct ttagagactt tacccagagc tgaaaagaca gattttgcag
      541 caacattaat cagaactgct aacaaaaacg aagctttgct cgaagcgaag atccaaaagc
      601 gtggacaaaa aaataagaag aaaaacgcgg gaagagtgag taagcaactt atagatgaca
      661 gttctttaac aagtaggaaa gctattcgtg ccttaaatat taccagtagg ctcgatggta
      721 agagggagaa agccattcat agagctaaat atgtgcagtc agctcgtaaa gctggatggg
      781 atactacaaa ccaaagcatt aggaaagaat tatcgacact acaaaacgca gcaaatacgg
      841 tcagcgattc cgttaacaaa agcacggaca aggaagggtc tgcgaatact gaagatgcga
      901 tgatagaaga cgatcaagat cacattgaaa tttacgacag tgctaaaagc cagaaggtac
      961 aagaaaaagc agtatcaacc acttttacgg catcaaacgt atttacgtca ttagaggatg
     1021 agatgaattc gcatctagac tgatgagacg aacgtgagac ctgcatagac caataaaaaa
     1081 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1141 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     1201 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     1261 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     1321 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     1381 aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat
     1441 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     1501 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     1561 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     1621 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1681 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1741 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1801 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1861 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1921 aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1981 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2041 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2101 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2161 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2221 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2281 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2341 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2401 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2461 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2521 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2581 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2641 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2701 aaccgtagca
//
