LOCUS       sequence-244840-        2665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P4
FEATURES             Location/Qualifiers
     source          1..2665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1030..1064)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1135..1794)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1709..1728
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1976..2564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2465..2484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgaactaca ctcctatcgc acaggaagcc aggactccga acagctagct
       61 gcttgcttgc tgctcgcttg ttgcctgctt gctgcctgcc tacaaaccaa ccaaccttaa
      121 aacgctctct gttgccttct accaccgcac cagcccagaa ccccacgcac caccagtggc
      181 accatctccg agaatcgtcc ctcccccgga cgtccttaag tcataccact gcaaccactc
      241 aactctgaaa cgcacctggc ttccactttt ctgcacaggc tcccagctac cacatttccc
      301 cagaaattct cccatccatg cccctacaat taaaaatcac cttcycacaa ctccgcagag
      361 aaccagagca catgattggc atccctagcc cccatcacta ctggattctc ggttgttaca
      421 ctgcaacgtg cattcttcta tgccactcta cgctgtggtt tcctagtgtt tccaaaccag
      481 atttcttggt gtttcgtggc gatttcctga tttcctgatt tcctggtttc ctgattcctt
      541 ccttccttcc tggtggtgtc tctctttctg tgtgtttttt ctttctttct ttctttattg
      601 cttgcttgct gccatgctgc taccttaatt cttctttttt cctwccctaa ttcttgtaat
      661 ttgtaatgaa agacaagacc accatacata cacacataca tgatatcgtg gcattgtgcc
      721 atggcaccat ggaatgggat atgggatata tggcaccgtc tttacttagc tccgaagctt
      781 tgaggatccg aaatttggta tataaggggt ggaacattgt cagaaagaag caatccatcc
      841 aagttgtctt gatgtcagtt ttagtttaga tgtttcagca tttagtttta gtgttagctt
      901 gtctactctc cccaaggaac aaggacactt tttcatacaa agtcttctgt ttgcacgaat
      961 ttaattgcgt acgttctccc ccacacacaa ctcaactcag ataattttaa aatatgtgag
     1021 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1081 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1141 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1201 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1261 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1321 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     1381 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1441 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1501 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1561 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1621 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1681 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1741 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     1801 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1861 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
     1921 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1981 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2041 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2101 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2161 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2221 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2281 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2341 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2401 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2461 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2521 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2581 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2641 cccctgattc tgtggataac cgtag
//
