LOCUS       sequence-244846-        2405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P1
FEATURES             Location/Qualifiers
     source          1..2405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(770..804)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(875..1534)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1449..1468
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1716..2304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2205..2224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgccagtca cgtgtgaaac acgtgactat acttaccctc acattaccct
       61 cactctttca cattaccctc cccaccatat cttccccccc cagcttcctg tcttccacta
      121 tccctgcaac caccaccacc acacaacaaa cagccccaaa caacccccaa cagcccggaa
      181 aaatccacgc ctcctcccga acacacgaag cccgccccgg cttccactcc aacactgcca
      241 acactggaac cccgcaccac gtaaccaccc actttagtgg ctgcccgccc ctcctgcgct
      301 tcccttgcgc ttcccttggg accacactcc ggtggcatgg ccctcgcttg ctacatgcta
      361 cctttttttt gcccttttct ggccttttct ggcttttctg gcctcaccac agtgtagtgg
      421 tgtaccacag tgtaatggtg taacattgtc gaatggtatt gtagcctgtt gtagcctgtt
      481 gtagcctgtt gtagcctggt gtaatgtctg gcatagtctg gtgtctggtg tctggtgtct
      541 tttggaaact gaacagtgga aagtggaagt gaaaaattgt ataaatatag gtgtcccatt
      601 cgtggtttgg tggatgtgtc cttgacattg caggtttctc tctgtttcat tgtgttaaga
      661 cttgttaaga cttgataagt atttccctag taatacccca ccaaccatcg gttgatacat
      721 tttaatacac actacctata taaagataca aatatgtgag accagaccaa taaaaaacgc
      781 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      841 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
      901 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
      961 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1021 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1081 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1141 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1201 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1261 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1321 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1381 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1441 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1501 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1561 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1621 ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga
     1681 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1741 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1801 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1861 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1921 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1981 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2041 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2101 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2161 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2221 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2281 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2341 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2401 cgtag
//
