LOCUS       sequence-244848-        2403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P11
FEATURES             Location/Qualifiers
     source          1..2403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(768..802)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(873..1532)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1447..1466
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1714..2302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2203..2222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgggtgatt cttatatatg gtatacaatg taaactatcc agtaagtaat
       61 ggaggattga tatgttcttg ttctgtattg ctgcctttat atatggtatt gtacaaggta
      121 ttggagaaat aaagagtgga tgcaatggta ccagaggtgc cagaggtgcc agaacggtgc
      181 cagaaagatg ccagaacggt gccagaacgg taccagaggt tcgggggtcc cagagggtcc
      241 cagatggtcg tggttagccc tgtgaggcct tgtcaggcct tgtgtagctg gtttatagtg
      301 cggttgccca gagaaacggg gttacccggg ggcaattctt ccatgctgcc accttcccat
      361 gcgaaccctc tggcacgacg cgaaacgcga aaaaaacaaa gccacgcgtc gtgccagggc
      421 gttcccccgt gaaacttaca gcacgacagc acgacagcct gcttctccat agtgtagcga
      481 cggccggtac cacgtccatt ccagctggtt ccatgggcct gaaccgagcg gttttcccgg
      541 gccctgtggc gtgcgaaact ggtggcatgg tggaggaaat tatatattgg atattccttg
      601 gatattccac tatctttccc tgtgtcatgc catatataaa gacaagagtg ttgcttgaga
      661 gttgatacta gacacttgat tctacttaga acatgttatt aaagtataat accccccaca
      721 acaagcaata aacagcaatc aataaataaa tatgtgagac cagaccaata aaaaacgccc
      781 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
      841 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
      901 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      961 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1021 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1081 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1141 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1201 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1261 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1321 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1381 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     1441 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     1501 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     1561 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1621 cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1861 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2401 tag
//
