LOCUS       sequence-244850-        2296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P17
FEATURES             Location/Qualifiers
     source          1..2296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(661..695)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(766..1425)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1340..1359
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1607..2195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2096..2115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acggtttcca gtgtgtgatt cccccagtgg aagcgcgtcg aaagaggagt
       61 cgtgctacgg ccggagcact atcttccggc cgccgcccca cccaccatcc tcgacactct
      121 ctgcatatgg gcagtgtgat ctgtgtttac ctatattacc tgtggtaagt agcacacaca
      181 tacatacaca ccagatttct ttggagaacg agcacattgt ttcgtctggt gttgtgattt
      241 gcttgggttt tctttcgcct gttgattgcc ctgtgttttt gactttgtct ccaagggcgt
      301 gcgcacccac ctggacttaa gggacaatct ggcagaggca gaggcagagg cccaggcatt
      361 gctctgggcc tgggcctggg cctggaacta tgcctgggcg cagcccaggc attggttggt
      421 ttaggccctt cccacccact cccatatcaa acccatatca accgcaattg ctctcgtgaa
      481 ggtttgtatt ttgcaatttt ctgtataaat agggctggca tggaattgtt acagcaatct
      541 ggtcagtcag tccccttact acttgctagt agatgtaata ctggttgtaa atagttctga
      601 cagtagtgtc aattatctcc accaaaacac agtatagaaa cactatgtga gaccagacca
      661 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      721 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      781 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      841 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
      901 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
      961 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     1021 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1081 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1141 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1201 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1261 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1321 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1381 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1441 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1501 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtag
//
