LOCUS       sequence-244869-        2513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus right homology arm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I3R
FEATURES             Location/Qualifiers
     source          1..2513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(878..912)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(983..1642)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1557..1576
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1824..2412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2313..2332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtaattcaa ggttgttcaa tattccgatt gttagtagat tcagttctac
       61 cttcgctcag ttttcttaat gactcaaagg atattagaat tgaaaaattt caaaaaagct
      121 ttaagcatag aaccacgcac ttattaaata caatggagaa atgatttggt gggcaattac
      181 cggtttcaat tgactccgaa caggttcaaa tacaccacat ttaacagact actaagaaag
      241 agtcaaaaag atttggctga acattaaggg aggctaggtt ttttagtgac tttttgcgag
      301 agaaacgctt ttttagcaga ttaagcaata agtttaaaca tgtccgttga attgtccaaa
      361 gtatccatct tgggtcagga caccattcat gtcggatacc agattcagga acacattgtg
      421 aagactactc tggaacaatg caaatcgtcg acatatgtta tcatcaacga tactaatgtc
      481 taccaggtgc cttactacca tgacttggtt gagctatttg agaagtctct acccgagact
      541 tcccgtttgc tcacatatgt tgtgaagcca ggcgaggcta acaagacccg tgaaactaag
      601 gcacagattg aagactttat gttgaatgaa gggtgcactc gtgataccgt cgttattgca
      661 attggtggtg gtgtgatcgg tgatatgatt gggtttgttg ctgcgacatt catgagaggt
      721 gttcgtttca ttcaaattcc tacatcgcta ttggcaatgg ttgactcgtc tattggtggt
      781 aagactgccg ttgacacccc aatcggtaaa aacttcattg gtgccttctg gcaaccacaa
      841 tttgtgttgg cgcgcctcag ccgatgagac cagaccaata aaaaacgccc ggcggcaacc
      901 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
      961 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1021 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1081 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1141 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1201 tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1261 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     1321 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     1381 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     1441 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     1501 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     1561 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     1621 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     1681 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     1741 cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1801 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1861 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1921 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1981 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2041 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2101 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2161 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2221 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2281 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2341 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2401 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2461 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tag
//
