LOCUS       sequence-244872-        2557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus right homology arm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I1R
FEATURES             Location/Qualifiers
     source          1..2557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(922..956)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1027..1686)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1601..1620
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1868..2456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2357..2376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agttgcttaa ttagcttgta catggctttg tggtttctgc cataacaagc
       61 ttcgttaccc aaggaccaga taataatcga aggatgattg acatctctta ggacaagttg
      121 ggaagctctg tctaagtacg cgacctcgta ctctggatta tctgataagt aatgggcatt
      181 aacatcgtag agtttatttt tggtatctgg atattcagcc tccaagttcg tatgacgatt
      241 aaatggctct tgaacaccgt gagtttcaag atctgcctca tcaatgaccc agaagcctag
      301 tttatcgaag aggtcataca ccttaggatg gtttggataa tgcgagttac gaacagcatt
      361 gatgttaaac ttcttcatta gaatcaagtc tctaacaaca aaatctaatg gcacagctct
      421 accgaacctt ggatggtgat catgcctgtt gacacctcta aagagaatgt ctttgccatt
      481 aacagtaatg ttaccgtcct tcaactccac ttgtctgaaa ccaacatggt gcttaataga
      541 ttgaatcaca ctgccatcag atccaattaa atccaactgg tacttgtaca aagtaggatt
      601 ttctgcggtc caatgttctg gggccttgac gttgatcttg aaagctgttt cttcgtcctt
      661 tttggtcgaa aaggaaataa attctttagt tgaaaacgtc gtgttcccat tctcctcgtt
      721 caacaaagag cttgcatcgt aaactttaga tccatcttca ggttcgtaaa gtgtgaaatt
      781 gatgtgatca taagaagaac cctggacatc aactttcaca gaaagctctg catcctgata
      841 ctgagagtcc acaaaagttg tagtgaccct aacgtcttca atatgggcct tcggcgcgcc
      901 tcagccgatg agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      961 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1021 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1081 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1141 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1201 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
     1261 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1321 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1381 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1441 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1501 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1561 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1621 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1681 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1741 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
     1801 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1861 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1921 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1981 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2041 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2101 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2161 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2221 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2281 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2341 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2401 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2461 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2521 ttcctgcgtt atcccctgat tctgtggata accgtag
//
