LOCUS       sequence-244874-        2561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus left homology arm; type 1 part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I4L
FEATURES             Location/Qualifiers
     source          1..2561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(924..958)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1029..1688)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1603..1622
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1870..2458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2359..2378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcac cctttcaggc gcgccggtgt tgcattagta tcgccgtgga tgaagatgaa
       61 ggtggaatag acgttaccca tagatacccg aatgagtatc tgattgagga tttttgactt
      121 acttacttct tcgagataca ctactctgcc gaaatttcgg actggaatgc gtcaaagtga
      181 atccactttg caggaaaacg caggaaaaac ggattcgaaa acgcaacaaa aaaaaaaaaa
      241 ttatacataa taaaatttga atcaaagcaa caagttcacg aatccattaa agcagtcaga
      301 atccaggaaa aaaaagaaac agagccagaa aaaaggcagc aacgaatcac aaccacagcg
      361 tattgcgtca gtggaaatta attaccttgg tatttccaat gctgtgtaca accaaaattt
      421 ccacatagtt agttgattcg catttccctg gtaaccgccc tcggcaacct ccctcggaaa
      481 cttccctcca gagtaacttc ccccagagtt atagagatat agagttatag tagagatata
      541 gagctataga tagattgatt cttgttgtag catttattcc gttaaacttc tgcggctaat
      601 tttgcctagc ttggcctttt ttttttgttt ttcgagcttt caaagacaaa tggacattct
      661 tacataatat attatcatat acaccgagat cggtgggtca gagcctgtag ggatggacat
      721 tttccttcta gactcgcctt ccagaccttt ttttggtttg tttctctctc cgtggaactc
      781 gctttctttt tcgccggtga tctcgaaacg taatcgatat gtattccatt ccattcgcta
      841 ttttttttgt tccataaacc atgttcttat gaacagaatt cgcatctaga ctgatgagac
      901 gaacgtgaga cctgcataga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      961 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     1021 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     1081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     1261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1441 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
     1801 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1861 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1921 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1981 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2041 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2101 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2161 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2221 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2281 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2341 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2401 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2461 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2521 ctttcctgcg ttatcccctg attctgtgga taaccgtagc a
//
