LOCUS       sequence-244879-        1919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus terminator; type 4 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.T2
FEATURES             Location/Qualifiers
     source          1..1919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(284..318)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(389..1048)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     963..982
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1230..1818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1719..1738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccgaatttt atacttagat aagtatgtac ttacacatag atttctatga
       61 gatactgatg tatacatgca tgataatatt taaacggtta ttagtgccga ttgtcttatg
      121 cgataatgac gttcctatca aagcaataca cttaccacct attacatggg ccaagaaaaa
      181 tattttggaa cttgtttaga atattagcac agagtatatg atgatatccg ttagattatg
      241 catgcttcat tccgacaact ttttcggctg tgagaccaga ccaataaaaa acgcccggcg
      301 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
      361 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
      421 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
      481 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
      541 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
      601 agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
      661 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
      721 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
      781 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
      841 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
      901 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
      961 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     1021 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     1081 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     1141 cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtag
//
