LOCUS       sequence-244894-        2565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P13
FEATURES             Location/Qualifiers
     source          1..2565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(930..964)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1035..1694)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1609..1628
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1876..2464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2365..2384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtagatta ataaaatata tcttgcgttt caatactcta gtctaacaaa
       61 agcgtacgta agtctttctt tacgtttgtt ctttgttctt tgttttccat ttttccataa
      121 ttaaaactga cgcccgacgt gcggccggcc ggcgcgtcca cagttgacac aaaactactc
      181 gcacgtgacc gcacgtgacc gcacgtgacc acattcatcc attcttctcc agaacattct
      241 agaaggaccg ggtaacgtcc acttccttcg aaacccggaa gaaagaaaca aagaaaaaaa
      301 aaaaaacctt tgcggacgtt aatatggagc atcatcgaaa taaagggaaa cgaagttgca
      361 ttaggcaaat agtctccagt ttcgttaaaa catcgcaacc ttttggaagt ggtaaggttt
      421 agcgcatgtt ctttgaaaaa ggcgcggttc tagtaggtgc tagtatcgtc tagaagtggg
      481 ctgtccaata gggagtgtcc ctaattttgc agtttgtagc cttacgatat gtggggcatt
      541 cgtagggttg gtctacacat gttgtgtgtg tgtgtatgta gtttagctgt gtagtttggt
      601 cgtaaggtga ctaaagaagt aactagcgag cggattgttg tagtttaggg tagtgtgaat
      661 tgcttcaagg gaaaatgggt ttgttctagg gaagaaaaga aatatgcaga acaaagtgaa
      721 ggaaacgaag tgaaaaggtt aggttccgga aggaagtata taaaaagggt atccagtgca
      781 agggaacgag aatagtttta gttcttggct aaggtatcta ggaggaattg ttgttattgt
      841 tgttgttgtt tattaattag tcacttccta gctcagacta gtaaagaaga tacaaaccat
      901 atagtgaaca aatatgtgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      961 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1021 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1081 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1141 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1201 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
     1261 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1321 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1381 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1441 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1501 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1561 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1621 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1681 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1741 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
     1801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1981 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtag
//
