LOCUS       sequence-244895-        2392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P3
FEATURES             Location/Qualifiers
     source          1..2392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(757..791)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(862..1521)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1436..1455
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1703..2291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2192..2211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgaagcctt gagcttcgtg tttattcact ctgtacgggc ggttctagcg
       61 tgtctgggaa tccaaagagg aagggccgtg tttcgtgcag gcattttagt ggatgcccgg
      121 gatgtaagct attcggttac agaaaatcag gttgtgattc cagtatgagg aatagtggat
      181 gccaggttgt gcgagcatca ccaatatcat gggaaatgcg aaatgggcat gagcccaacc
      241 aacacaacac aataccatac gccaaatggt agcctgaaaa aaaaaaaaaa ggtcttgcca
      301 gaccactaaa ctcgaggtag aacaagactg agaaagagtg tgtgatccct ttggtggtag
      361 taattttttt tttttttttt ttcaagtttc cagcatccca aaccgaaacc caacatcatc
      421 atgatcccat cccatgccta tgcccatgcc acattcattc attgcacaac acacaacgta
      481 gtggacgaca gcatagcatc agttaactaa cggccacttg tttttttttc ttccattttt
      541 tcgttttgtt ctagcaacaa tgagttctaa tttttttgtt ataaaaggga cagttaaggt
      601 atatgtaagg tttcttgttc catttgggtt agagttttgt agtattagtt ttggttctta
      661 gtccttttgg cctaagaaac cagtaagaat tcttctgatt ttctttacaa acacatcaac
      721 aacaacaaac acaaactact atgtgagacc agaccaataa aaaacgcccg gcggcaaccg
      781 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      841 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
      901 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
      961 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1021 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1081 ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1141 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1201 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1261 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     1321 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1381 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1441 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1501 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1561 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     1621 gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt ag
//
