LOCUS       sequence-244898-        2559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus left homology arm; type 1 part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I3L
FEATURES             Location/Qualifiers
     source          1..2559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(922..956)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1027..1686)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1601..1620
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1868..2456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2357..2376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcac cctttcaggc gcgcctcata ccgtttatga ttgcatttac gttactgacc
       61 ggagttttgt ccgtatttgt gaagccgttg gagataattg aggcaccaag gttcgaacca
      121 gactatgcta tattctaccc aggaattaat tgccagtcat gccatgctgt taaagtaccc
      181 cgttcgaaac actgttcact gtgtcggcga tgcattccat tgcaagatca tcactgcatt
      241 tggatcaaca attgtgttgg ctacggtaac tatgaatact tctatccctt cctactagcg
      301 aactgtacca ttttatcgta tgcatccatc agactatttt caatgttgag agttctgcga
      361 gttgtgagca aacatttcct gtctctcttt ttgttaacaa cagcgtttgc attacttgcc
      421 ataatattta catactacca gttaaagctc gttcaagaag gaatgaccaa caacgagcaa
      481 gataaatggt acctcgtaca tgaacatatg agggatggaa accttttcaa ggataacaat
      541 ggctcactgt actacagaac cagttctggt gtggaaggaa ttgatgacac attttactct
      601 accaacttgt acgatcacag aaagtacaaa ctcaacaatc cttcgagaat acacagcgcg
      661 gaagaactca caaatattta tgatagagga gacttctgga agaaccttgt agagaggata
      721 agtgtccttt gaaaatctga ctttaagaat tctcgtagct ccatgtacat aataaatata
      781 gactaataag attcgcataa caaactggct tcaaatcata atggcaaaga atcacaaaat
      841 tcgagatttc aatctagcta atattcctaa ccggaattcg catctagact gatgagacga
      901 acgtgagacc tgcatagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      961 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1021 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1081 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1141 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1201 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
     1261 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1321 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1381 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1441 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1501 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1561 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1621 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1681 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1741 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
     1801 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1861 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1921 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1981 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2041 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2101 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2161 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2221 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2281 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2341 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2401 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2461 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2521 ttcctgcgtt atcccctgat tctgtggata accgtagca
//
