LOCUS       sequence-244899-        2589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus left homology arm; type 1 part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I1L
FEATURES             Location/Qualifiers
     source          1..2589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(952..986)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1057..1716)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1631..1650
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1898..2486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2387..2406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcac cctttcaggc gcgccgaaag tgattgaaga accctcaaac ttgctggata
       61 tttccttacc cttgaccttt aggctttcaa ttttacccaa caatttgtcc aagacaaatt
      121 gcaatccact ggattcaact gagacataac gtttaccgtc gttgatcttg gtaggttttt
      181 ctgctgtctc tgtaacaaaa tctggtacct ttaatggaag ttcggcttgg ccccaggcaa
      241 tttcatgacc tgcctttaga acaccagcat catcctttaa cacggcaaca acataagtgg
      301 tatcagaagg aatagtaaca gattcttctg gctttaaagc tggaacgtcg attgtctttc
      361 ccgtgtcctt gtcgataaac aataagtgat ctgtcgtaat gaagtcgtgc ttatttgtga
      421 ttgttacaga tccgtgcgca attttaatat gaacgggttc aataaccttc ttatactcta
      481 caaggcccgg agtaggatta tgctcactgt tacacaaacc atccatgatg aacactccgt
      541 catgaacctc ttccttaaag tcaccaccat aagcataagc tttatgcaac ttaccatctg
      601 cagtactaac atcttcgaat tcaataccgt gatttgccca ttcccagata aagccacctt
      661 ggtaaaactt ctccttgtag aacaactctt gatattcttt caaagaaccc gggccgttac
      721 ccattgcatg accgtactca cacaagatca aaggcttttc aaacttacca ttttcatcag
      781 tgtggttctt cctccacctt tccataattt caaatgttgg gtacatgaaa ctaaagatat
      841 ctgcactcaa agcgttcaag tcaccctcat aatgcaccag tctggtagga tccaattgct
      901 taagaattcg catctagact gatgagacga acgtgagacc tgcatagacc aataaaaaac
      961 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1021 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1081 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1141 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1201 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1261 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     1321 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1381 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1441 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1501 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1561 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1621 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1681 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     1741 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     1801 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
     1861 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1921 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1981 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2041 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2101 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2161 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2221 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2281 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2341 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2401 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2461 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2521 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2581 accgtagca
//
