LOCUS       sequence-244900-        2057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus centromere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.C1
FEATURES             Location/Qualifiers
     source          1..2057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(422..456)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(527..1186)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1101..1120
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1368..1956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1857..1876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtttctacg caagacttct tgaagtgaaa accaactttc agtcttcaaa
       61 ctaaaaatga aaatcagtgg gctaaattca gcataatacc tgtggatata attacataca
      121 aaaatataat taaaaatata cgttaaaaat aatttatttt tgtaaaaact ataaaatata
      181 ttttactttc ggaacaactt tttaacttat aattttgttt taaataaaaa cattgtattt
      241 aaaaataata aattattaaa taaaatttta aagtatgttt atttaaaata tagaatacta
      301 ttaaaattac tttaaacttt aaaataaaaa aataataaat tatatagttt taaaattaat
      361 aaattttata cacgtgacca gatcataata gttttctttc cttgccgatg agaccagacc
      421 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      481 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      541 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
      601 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
      661 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
      721 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
      781 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
      841 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
      901 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
      961 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1021 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1081 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1141 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1201 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1261 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1501 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1981 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2041 tctgtggata accgtag
//
