LOCUS       sequence-244902-        2633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P12
FEATURES             Location/Qualifiers
     source          1..2633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(998..1032)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1103..1762)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1677..1696
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1944..2532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2433..2452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgctagcct ggaccgggat ttccctcact acgcagcagc tagctcgctc
       61 gcttagcgcc tggagttcca gaatcttccg aaatcctgac taaaagacaa gaaaacaaag
      121 tacgggtaac actaatctat ttcgcagccc ttcgctcttc tgtttgcagc taattaatta
      181 attagccaat ttttcattgg gaaaaaatcc tgattgtgca gatgttgaaa atatatataa
      241 agggaggcaa catattttga accaacatta aggatttcca ttgccactgt agtaaaacta
      301 gttggattga ttagtttaga ctttgcaatt gctttctaaa atactcaatt accacaccag
      361 taatcccata actaacatca attaaaaatg gttgcccaag tccaaaagcc agccccagag
      421 ttcaagaaga ccgctgtcat tgacggtgtt ttcgacgaag tttccctaga aaaatacaag
      481 ggtaagtacg ttgtcttggc cttcattcca ttggccttca ccttcgtgtg cccaactgaa
      541 atcattgcct tctctgaagc tgccaagaag ttcgaagaaa ttggtgctca agttttgttc
      601 gcttccactg actccgaata ctccttgttg gcatggacca acgttgctag aaaggacggt
      661 ggtctaggtc cagtcaacat tccattgatt gctgacacca accactcctt gtccagagac
      721 tacggtgtct tgatcgaaga agaaggtatt gccttgagag gtttgttctt gatcgatcca
      781 aagggtattg tgagacacat caccatcaac gacttgccag tcggtagaaa cgttgaagaa
      841 gctttgagat tggtcgaagg tttccaatgg accgacaaga acggtaccgt cttgccatgt
      901 aactggactc caggttccgc taccatcaag ccagacgtcg aagcttctaa ggaatacttc
      961 gctgccgcta acaaggaata tatgtgagac cagaccaata aaaaacgccc ggcggcaacc
     1021 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     1081 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1141 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1201 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1261 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1321 tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1381 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     1441 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     1501 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     1561 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     1621 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     1681 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     1741 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     1801 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     1861 cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tag
//
