LOCUS       sequence-244903-        2640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P10
FEATURES             Location/Qualifiers
     source          1..2640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1005..1039)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1110..1769)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1684..1703
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1951..2539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2440..2459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgttttgaa cgcgaggctt tttccacttt gacacatttt ttcttagtga
       61 aaactgaaga aagaagtagt agggttgaga gtagagtaga agtggaatgt caataatgac
      121 tttagcaggc tctaaagcaa aaagaaaaca gacaaaccaa aattaaaatg ccttcaaaat
      181 cactcaatat gtaaacaaac acccaattaa ttacatacgc attactgtcg ctacgatact
      241 ccactattgc gcttctccga acctcaatgt tattgtttgt cccacacagt catgatcaag
      301 ttctaagtac tgatcattaa ctaaacaaaa tcactaacaa cacacactag aaagtcctta
      361 catagaaatt cccgaagaat gtcaaagcgc ttctatttac ttactcaact gttcacttcc
      421 gacaaggatg gaaggggaga aacaatagtg aaaaaacata tataaaggct gtgtaacatg
      481 tgctcggaaa gaaaacatgt tgggattaat tattactctt gttctataat taggaatttg
      541 ctgtccataa acggtatagt aactgaaaga cttctcacta tttaattggt caatatccaa
      601 ttggtttaaa gacagacaga ccacaaatac gaaaatttaa tttaaccgcc ttgctagcaa
      661 ctctaatcct gttcggagta tagtgaaata agcaacgacg aaaaaacaca cacacgcgct
      721 tacacaacat tagtgaaaac gaagtaacat ttattactta attactatag ttgaaaaaag
      781 aaccgaaaat agcaggcacg accaacaaaa gctactttct aacagacaag gacatattaa
      841 tcaatttcga atatttagta tttcctctaa cacaataaac actccccctt ccttattggc
      901 ccctcatttt caaagagtaa gacaaaaatc aagaggaaaa ttaacgcccc agagtacaat
      961 atcataatat caaaaaaaaa attctactat gtgagaccag accaataaaa aacgcccggc
     1021 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1081 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     1141 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1201 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1261 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1321 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1381 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1441 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     1501 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1561 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1621 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1681 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     1741 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1801 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     1861 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
//
