LOCUS       sequence-244904-        2924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus centromere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.C2
FEATURES             Location/Qualifiers
     source          1..2924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1289..1323)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1394..2053)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1968..1987
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2235..2823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2724..2743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtgagctcc tttcatttct gataaaagta aggtttctct atttatcttt
       61 tcacccacat tatccttcga agtacgtata caatattagt tcaacgtaaa aacaaaactt
      121 actgtaaata tgcgtaaaaa aaatctatta aattcatagc agtttcaagg aaagagaacc
      181 attatggtct ggtcacgtgt gtataaatta ttaattttaa cactatataa tttattattt
      241 ttttattttg aagtttagag taattttagt agtattttat attttaaata aatatatttt
      301 aaatttttac ttaatatttt attatttttt aatacaatgt ttttatttaa aacaaaatta
      361 taaattaaaa tgttgttccg aaagtaaaat atattttatg gtttttacaa aaataaatta
      421 tttttaatgt atttttttaa ttatattttt gtatgtaatt atatccacag gtattatgtt
      481 gaatttagct gttttagttt acctgtgtgg tactatgagt tttttgcctc tcaaaagcta
      541 ttttttagaa ctctctctcc tcttagaaat aggtggtgtt gcggttgact tttaacgata
      601 tatcattttc aatttattta ttttaaagtg acatagagag attcctttta attttttaat
      661 ttttattttc aataatttta aaaatggggg acttttagat tggaacaaaa tgaaaaatat
      721 ctgttatacg tgcaactgaa ttttactgac cttaaaggac tatctcgaac ttggttcgga
      781 aatccttgaa atgattgata ttttggtgga ttttctctga ttttcaaaca agtagtattt
      841 tatttaatat ttattatatt ttttacattt ttttatattt ttttattgtt tggaagggaa
      901 agcaacaatt actttcaaaa tatataaatt aaactgaaat acttaataag agacaaataa
      961 cattcaagaa tcaaatactg ggttattaat caaaagatct ctctacatgc acccaaattc
     1021 actatttaaa tttactatac cactgacaga atatatgaac ccagattaag tagccagagg
     1081 ctcttccact atattgagta tatagcctta catattttct gcgcataatt ttctggatgt
     1141 aaaataaaca aaaatagtta gtttgtagtt atgaaaaaag gcttttggaa aatgcggaat
     1201 acgtgttatt taaggttaat caacaaaacg catatccata gtggatagtt ggataaaact
     1261 tcaattgatc cccgatgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1321 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1381 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1441 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1501 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1561 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa
     1621 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1681 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1741 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1801 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1861 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1921 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1981 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2041 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2101 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc
     2161 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2281 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2341 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2401 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2461 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2521 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2581 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2641 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2701 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2761 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2821 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2881 atgttctttc ctgcgttatc ccctgattct gtggataacc gtag
//
