LOCUS       sequence-244905-        2440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P14
FEATURES             Location/Qualifiers
     source          1..2440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(805..839)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(910..1569)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1484..1503
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1751..2339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2240..2259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgactatct acacattttg taaccaccct ctgctctgct cattcttcta
       61 cttcttaaaa atacgatcaa tctatatcaa aagcatgtat tttaccaaac gcagtaatta
      121 tagcgaccat ttaacacata tagtaactaa tggcgctaat gacataattt gtccgaaaga
      181 attcgccttg ttggcatttg tcctttctag gcattaatag cgcttccttt tctggccctt
      241 accatattct aagtgtcagt caagattgcc atatacttgc tgtataattc aatttcgtca
      301 ttcttgtcgt ttcgtcatat ctgctaatcc attcgttcag ccaactgctt acaattactc
      361 agatgctcgt aagaaaaact caattgcgtt tttttgccgc tagaagtgta tctgttcaga
      421 tctatagaat tttctagaag taaacgcccc tacaaaaatt ttcgctcgaa acagaacgtt
      481 gaaaagaggg aaaaaaaaaa tctatagatg gacgagcgag aatcatatat aaagcggcga
      541 gttcggtggc aactcgaacg taaagagcta aaagttttac atgtatagga aatattgggc
      601 caattgtatt caattgtagg tttttttata ggtattttag cttttagagt aagaaagtcg
      661 acgcgctagc tagtttagga tcgttagtca gaagttgtat acatacaata ccttcagaaa
      721 gaacacctgt cactctctgg cattacgaaa gaaaacttca aaaaagtcaa ttaataaatt
      781 aagcaaatat gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      841 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      901 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      961 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1021 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1081 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
     1141 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1201 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1261 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     1321 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     1381 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     1441 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     1501 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     1561 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     1621 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
     1681 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1861 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2221 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2281 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2341 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2401 tctttcctgc gttatcccct gattctgtgg ataaccgtag
//
