LOCUS       sequence-244906-        1898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus ARS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.C4
FEATURES             Location/Qualifiers
     source          1..1898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(263..297)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(368..1027)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     942..961
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1209..1797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1698..1717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtcaatact ttctaacatt gaccattttg catcattttg ttacggaaaa
       61 gtgctttcca atcatatata attaaaacta gcggatcaca ttaaaaagtt catccaaagt
      121 gaaaaaacat tctaaattta gtagacagca attgttttaa aagggtgttc ctattttcat
      181 ataacggaga gagttaagct tcctgtatgc aattagcaaa tattttcact gtagagttct
      241 gggacccgat gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      301 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
      361 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
      421 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
      481 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
      541 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
      601 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
      661 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
      721 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
      781 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
      841 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
      901 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
      961 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1021 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1081 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     1141 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1201 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1261 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1321 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1381 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1441 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1561 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1741 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1801 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1861 tttcctgcgt tatcccctga ttctgtggat aaccgtag
//
