LOCUS       sequence-244907-        1916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus terminator; type 4 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.T3
FEATURES             Location/Qualifiers
     source          1..1916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(281..315)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(386..1045)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     960..979
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1227..1815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1716..1735
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccatataaa aaccggtcac gtgagcgcga gcaatcgccg attaccctct
       61 cacaagggtc cttctacttc ctattctgtg ttccccgttc cccggttctt atattttccc
      121 tattactaca taatcaccat atataagatg cgatgattcg accctgatag acaagacaag
      181 acaggctctt caatgatata gagacacggt gtagccttct ttttttttgc gtgggtcatt
      241 gcaacagctt agctaacaac aacgctgtga gaccagacca ataaaaaacg cccggcggca
      301 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      361 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      421 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      481 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      541 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      601 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      661 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      721 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      781 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      841 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      901 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      961 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1021 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1081 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1141 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1201 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1261 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1321 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1381 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1441 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1501 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1561 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1621 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1681 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1741 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1801 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1861 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
