LOCUS       sequence-244908-        2645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P8
FEATURES             Location/Qualifiers
     source          1..2645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1010..1044)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1115..1774)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1689..1708
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1956..2544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2445..2464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtgctgga tggagaattt tcgcgggaaa accagaaaaa acacagaacc
       61 aaaatcagca gggtgcacgg gtccttgtgg gcgtccacaa tggaaggttt ggaatcaaac
      121 gagtcataca cagcagaaaa ggacgcacgt ccgcacggcc ctccggaaaa acattctgcc
      181 ggcaccatcg catcgatcca cgtagccact gactgagact gagactgaga ctggatctac
      241 ccgctggatc tgggtggtgt gttttttttt gttttgacat gattttttcc cacccaccca
      301 cgtcaacaac gtaaggctag gtcaggtata ggtaaagata taggtactat tttccctgcg
      361 gccccttctg tgccagcgcg gataatttag gttattccca gatgagcaat tttcccggaa
      421 aaactttttt ttcgtatttt tttgattttt ttcaattttt ttgaattttt tgttttggtt
      481 ttggtttctt gggcctcgga caacccgggt gggagcagcg cggcacgaat ctggcgcacc
      541 tcgcagctgg atcgcctctt cccttattct ctcgccagcg caggagcgca ggagtggcag
      601 gtactcccgc gcaggtactc ccgcaggcac tcccgcgcat agaactcgaa acaaatgtcg
      661 taccacaatt atgtttcggg gccctcctgc ctcacaccgc attgtccaac aacggtgctg
      721 caaaaagaaa gtgttctaga atgaggaatg ctgaaaagaa aagcgaacgg ttggcactgc
      781 atggcactgg catatcatat catactatat actatactat actatactat actatactat
      841 actatactat actatactat actatactat actatactat actatactat actatactat
      901 actcaaggct ttatcctatt accactcgct tgccctctta tgttcaggta caggcatttt
      961 cccatccacc cagttagtca gtgacaacct agtatgtgag accagaccaa taaaaaacgc
     1021 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1081 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1141 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1201 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1261 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1321 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1381 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1441 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1501 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1561 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1621 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1681 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1741 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1801 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1861 ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga
     1921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2101 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2341 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2521 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2581 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2641 cgtag
//
