LOCUS       sequence-244911-        2291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P18
FEATURES             Location/Qualifiers
     source          1..2291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(656..690)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(761..1420)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1335..1354
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1602..2190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2091..2110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcctgggg aaacggaaac cgaagaacat attgaaaaat aataccaaga
       61 ctcaaattca caagaacttt ttcgccgcat ggtaggctag atttcggtta gaaagtgtgg
      121 ggagatattt ctgaagatgt gctatactgt ggataaggtt tatgcaatgt atgtgatagt
      181 ttcgaaggcc aattagtgga gaacttgaca tgatgcaagt caaatgattg actaggcagc
      241 gctacaccat agtgatacat aattatttct ttttaatctt tacaccgtct tgtttgaggg
      301 ttgattggta ttaaaaataa aaaaaaaaaa aacacgcacc ttaatatatt attatcttgc
      361 atatataatc actacaagaa acgttttcga attggaatca tctcacttgt ttcaactaat
      421 acaatgcaaa gctggtttat gtaataataa gttaaaagtc tcattactag ctttttaatc
      481 tctttctttt tggtttatac tagcaaggat cgttggattt atatacatat ataaagcaag
      541 gtactaaatt caaattaggg tactattaag gttttctcat tttggaacaa gaagaaaagg
      601 agcgagcgct accaataaca aaaatacaaa ttatcaacta tgtgagacca gaccaataaa
      661 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      721 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
      781 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      841 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      901 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      961 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     1021 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1081 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1141 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1201 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1261 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1321 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1381 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1441 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1501 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
     1561 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1621 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1681 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1741 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1801 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1861 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1921 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1981 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2041 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2101 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2161 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2221 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2281 gataaccgta g
//
