LOCUS       sequence-244912-       12868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus delivery for stable expression of AsCas12a-VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Breinig M, Schweitzer AY, Herianto AM, Revia S, Schaefer L, Wendler 
            L, Cobos Galvez A, Tschaharganeh DF
  TITLE     Multiplexed orthogonal genome editing and transcriptional activation
            by Cas12a.
  JOURNAL   Nat Methods. 2019 Jan;16(1):51-54. doi: 10.1038/s41592-018-0262-1. 
            Epub 2018 Dec 17.
  PUBMED    30559432
REFERENCE   2  (bases 1 to 12868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0262-1"; journalName: "Nat Methods"; date: 
            "2019-01"; volume: "16"; issue: "1"; pages: "51-54"
COMMENT     Sequence Label: pMB22
FEATURES             Location/Qualifiers
     source          1..12868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    64..181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        299..510
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      553..562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             565..585
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             610..4527
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4247..4256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4528..4575
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4609..4758
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4783..4803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5233..5589
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5608..6177
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     promoter        6205..6704
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6240..6263)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6637..6656
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6725..7324
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6725..6744)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7221..7241
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7377..7965
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7430..7450)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7848..7859)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7968..7984)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7969..7985)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8494..8674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8736..9324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8816..8835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9495..10286,10287..10355))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10118..10137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10356..10460)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10535..10554)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10726..11105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11107..11305
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11260..11280
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11323..11503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11550..11675
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12172..12405
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12590..12634
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
       61 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      121 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      181 tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc cgggtcgact
      241 agtgatcagt gtgagggagt gtaaagctgg ttttcgagtg gctccggtgc ccgtcagtgg
      301 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
      361 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
      421 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
      481 tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac gcggctaact cgaacgctag
      541 ctgtgcgatc gtgccaccat ggccccaaag aagaagcgga aggtcggtat ccacggagtc
      601 ccagcagcca cacagttcga gggctttacc aacctgtatc aggtgagcaa gacactgcgg
      661 tttgagctga tcccacaggg caagaccctg aagcacatcc aggagcaggg cttcatcgag
      721 gaggacaagg cccgcaatga tcactacaag gagctgaagc ccatcatcga tcggatctac
      781 aagacctatg ccgaccagtg cctgcagctg gtgcagctgg attgggagaa cctgagcgcc
      841 gccatcgact cctatagaaa ggagaaaacc gaggagacaa ggaacgccct gatcgaggag
      901 caggccacat atcgcaatgc catccacgac tacttcatcg gccggacaga caacctgacc
      961 gatgccatca ataagagaca cgccgagatc tacaagggcc tgttcaaggc cgagctgttt
     1021 aatggcaagg tgctgaagca gctgggcacc gtgaccacaa ccgagcacga gaacgccctg
     1081 ctgcggagct tcgacaagtt tacaacctac ttctccggct tttatgagaa caggaagaac
     1141 gtgttcagcg ccgaggatat cagcacagcc atcccacacc gcatcgtgca ggacaacttc
     1201 cccaagttta aggagaattg tcacatcttc acacgcctga tcaccgccgt gcccagcctg
     1261 cgggagcact ttgagaacgt gaagaaggcc atcggcatct tcgtgagcac ctccatcgag
     1321 gaggtgtttt ccttcccttt ttataaccag ctgctgacac agacccagat cgacctgtat
     1381 aaccagctgc tgggaggaat ctctcgggag gcaggcaccg agaagatcaa gggcctgaac
     1441 gaggtgctga atctggccat ccagaagaat gatgagacag cccacatcat cgcctccctg
     1501 ccacacagat tcatccccct gtttaagcag atcctgtccg ataggaacac cctgtctttc
     1561 atcctggagg agtttaagag cgacgaggaa gtgatccagt ccttctgcaa gtacaagaca
     1621 ctgctgagaa acgagaacgt gctggagaca gccgaggccc tgtttaacga gctgaacagc
     1681 atcgacctga cacacatctt catcagccac aagaagctgg agacaatcag cagcgccctg
     1741 tgcgaccact gggatacact gaggaatgcc ctgtatgagc ggagaatctc cgagctgaca
     1801 ggcaagatca ccaagtctgc caaggagaag gtgcagcgca gcctgaagca cgaggatatc
     1861 aacctgcagg agatcatctc tgccgcaggc aaggagctga gcgaggcctt caagcagaaa
     1921 accagcgaga tcctgtccca cgcacacgcc gccctggatc agccactgcc tacaaccctg
     1981 aagaagcagg aggagaagga gatcctgaag tctcagctgg acagcctgct gggcctgtac
     2041 cacctgctgg actggtttgc cgtggatgag tccaacgagg tggaccccga gttctctgcc
     2101 cggctgaccg gcatcaagct ggagatggag ccttctctga gcttctacaa caaggccaga
     2161 aattatgcca ccaagaagcc ctactccgtg gagaagttca agctgaactt tcagatgcct
     2221 acactggcct ctggctggga cgtgaataag gagaagaaca atggcgccat cctgtttgtg
     2281 aagaacggcc tgtactatct gggcatcatg ccaaagcaga agggcaggta taaggccctg
     2341 agcttcgagc ccacagagaa aaccagcgag ggctttgata agatgtacta tgactacttc
     2401 cctgatgccg ccaagatgat cccaaagtgc agcacccagc tgaaggccgt gacagcccac
     2461 tttcagaccc acacaacccc catcctgctg tccaacaatt tcatcgagcc tctggagatc
     2521 acaaaggaga tctacgacct gaacaatcct gagaaggagc caaagaagtt tcagacagcc
     2581 tacgccaaga aaaccggcga ccagaagggc tacagagagg ccctgtgcaa gtggatcgac
     2641 ttcacaaggg attttctgtc caagtatacc aagacaacct ctatcgatct gtctagcctg
     2701 cggccatcct ctcagtataa ggacctgggc gagtactatg ccgagctgaa tcccctgctg
     2761 taccacatca gcttccagag aatcgccgag aaggagatca tggatgccgt ggagacaggc
     2821 aagctgtacc tgttccagat ctataacaag gactttgcca agggccacca cggcaagcct
     2881 aatctgcaca cactgtattg gaccggcctg ttttctccag agaacctggc caagacaagc
     2941 atcaagctga atggccaggc cgagctgttc taccgcccta agtccaggat gaagaggatg
     3001 gcacaccggc tgggagagaa gatgctgaac aagaagctga aggatcagaa aaccccaatc
     3061 cccgacaccc tgtaccagga gctgtacgac tatgtgaatc acagactgtc ccacgacctg
     3121 tctgatgagg ccagggccct gctgcccaac gtgatcacca aggaggtgtc tcacgagatc
     3181 atcaaggata ggcgctttac cagcgacaag ttctttttcc acgtgcctat cacactgaac
     3241 tatcaggccg ccaattcccc atctaagttc aaccagaggg tgaatgccta cctgaaggag
     3301 caccccgaga cacctatcat cggcatcgat cggggcgaga gaaacctgat ctatatcaca
     3361 gtgatcgact ccaccggcaa gatcctggag cagcggagcc tgaacaccat ccagcagttt
     3421 gattaccaga agaagctgga caacagggag aaggagaggg tggcagcaag gcaggcctgg
     3481 tctgtggtgg gcacaatcaa ggatctgaag cagggctatc tgagccaggt catccacgag
     3541 atcgtggacc tgatgatcca ctaccaggcc gtggtggtgc tggagaacct gaatttcggc
     3601 tttaagagca agaggaccgg catcgccgag aaggccgtgt accagcagtt cgagaagatg
     3661 ctgatcgata agctgaattg cctggtgctg aaggactatc cagcagagaa agtgggaggc
     3721 gtgctgaacc cataccagct gacagaccag ttcacctcct ttgccaagat gggcacccag
     3781 tctggcttcc tgttttacgt gcctgcccca tatacatcta agatcgatcc cctgaccggc
     3841 ttcgtggacc ccttcgtgtg gaaaaccatc aagaatcacg agagccgcaa gcacttcctg
     3901 gagggcttcg actttctgca ctacgacgtg aaaaccggcg acttcatcct gcactttaag
     3961 atgaacagaa atctgtcctt ccagaggggc ctgcccggct ttatgcctgc atgggatatc
     4021 gtgttcgaga agaacgagac acagtttgac gccaagggca cccctttcat cgccggcaag
     4081 agaatcgtgc cagtgatcga gaatcacaga ttcaccggca gataccggga cctgtatcct
     4141 gccaacgagc tgatcgccct gctggaggag aagggcatcg tgttcaggga tggctccaac
     4201 atcctgccaa agctgctgga gaatgacgat tctcacgcca tcgacaccat ggtggccctg
     4261 atccgcagcg tgctgcagat gcggaactcc aatgccgcca caggcgagga ctatatcaac
     4321 agccccgtgc gcgatctgaa tggcgtgtgc ttcgactccc ggtttcagaa cccagagtgg
     4381 cccatggacg ccgatgccaa tggcgcctac cacatcgccc tgaagggcca gctgctgctg
     4441 aatcacctga aggagagcaa ggatctgaag ctgcagaacg gcatctccaa tcaggactgg
     4501 ctggcctaca tccaggagct gcgcaacaaa aggccggcgg ccacgaaaaa ggccggccag
     4561 gcaaaaaaga aaaagggatc caaagaggcc agcggttccg gacgggctga cgcattggac
     4621 gattttgatc tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt
     4681 ggttcggatg cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat
     4741 ttcgacctgg acatgctgat taactctaga agttccggat ctccgaaaaa gaaacgcaaa
     4801 gttggtagcc agtacctgcc cgacaccgac gaccggcacc ggatcgagga aaagcggaag
     4861 cggacctacg agacattcaa gagcatcatg aagaagtccc ccttcagcgg ccccaccgac
     4921 cctagacctc cacctagaag aatcgccgtg cccagcagat ccagcgccag cgtgccaaaa
     4981 cctgcccccc agccttaccc cttcaccagc agcctgagca ccatcaacta cgacgagttc
     5041 cctaccatgg tgttccccag cggccagatc tctcaggcct ctgctctggc tccagcccct
     5101 cctcaggtgc tgcctcaggc tcctgctcct gcaccagctc cagccatggt gtctgcactg
     5161 gctcaggcac cagcacccgt gcctgtgctg gctcctggac ctccacaggc tgtggctcca
     5221 ccagccccta aacctacaca ggccggcgag ggcacactgt ctgaagctct gctgcagctg
     5281 cagttcgacg acgaggatct gggagccctg ctgggaaaca gcaccgatcc tgccgtgttc
     5341 accgacctgg ccagcgtgga caacagcgag ttccagcagc tgctgaacca gggcatccct
     5401 gtggcccctc acaccaccga gcccatgctg atggaatacc ccgaggccat cacccggctc
     5461 gtgacaggcg ctcagaggcc tcctgatcca gctcctgccc ctctgggagc accaggcctg
     5521 cctaatggac tgctgtctgg cgacgaggac ttcagctcta tcgccgatat ggatttctca
     5581 gccttgctgg gctctggcag cggcagccgg gattccaggg aagggatgtt tttgccgaag
     5641 cctgaggccg gctccgctat tagtgacgtg tttgagggcc gcgaggtgtg ccagccaaaa
     5701 cgaatccggc catttcatcc tccaggaagt ccatgggcca accgcccact ccccgccagc
     5761 ctcgcaccaa caccaaccgg tccagtacat gagccagtcg ggtcactgac cccggcacca
     5821 gtccctcagc cactggatcc agcgcccgca gtgactcccg aggccagtca cctgttggag
     5881 gatcccgatg aagagacgag ccaggctgtc aaagcccttc gggagatggc cgatactgtg
     5941 attccccaga aggaagaggc tgcaatctgt ggccaaatgg acctttccca tccgccccca
     6001 aggggccatc tggatgagct gacaaccaca cttgagtcca tgaccgagga tctgaacctg
     6061 gactcacccc tgaccccgga attgaacgag attctggata ccttcctgaa cgacgagtgc
     6121 ctcttgcatg ccatgcatat cagcacagga ctgtccatct tcgacacatc tctgttttga
     6181 cctaggaatc ccgaggcctc taccgggtag gggaggcgct tttcccaagg cagtctggag
     6241 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     6301 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     6361 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     6421 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     6481 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     6541 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6601 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6661 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     6721 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6781 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6841 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6901 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6961 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7021 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7081 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7141 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7201 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7261 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7321 ctgaaaacgt tgttgtttgg ggttgaatta ctcttctaga ccgcggcgcg cccgataatc
     7381 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7441 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7501 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7561 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7621 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7681 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7741 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7801 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7861 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7921 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     7981 ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     8041 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     8101 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8161 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     8221 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     8281 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     8341 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     8401 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     8461 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     8521 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8581 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8641 agaccctttt agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag
     8701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    10501 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10561 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
    10621 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
    10681 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
    10741 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
    10801 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
    10861 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
    10921 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
    10981 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
    11041 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
    11101 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
    11161 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
    11221 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
    11281 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
    11341 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    11401 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    11461 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    11521 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    11581 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    11641 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    11701 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    11761 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    11821 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    11881 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    11941 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    12001 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    12061 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    12121 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    12181 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    12241 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    12301 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    12361 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    12421 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    12481 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    12541 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    12601 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    12661 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    12721 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    12781 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    12841 agagagagac agagacagat ccattcga
//
