LOCUS       sequence-244913-        2529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus right homology arm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.I4R
FEATURES             Location/Qualifiers
     source          1..2529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(894..928)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(999..1658)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1573..1592
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1840..2428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2329..2348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtttcacaa tggagggagt gaaatctaaa agtggaaaaa aaaaggggag
       61 gcgaaaaaca aaaaaaaaaa aaagaacgaa ccgaactatg tccacaggag cagaagcaga
      121 agctgcggca gcagcttctg cggctttgaa agaggaaacg cgtagatgaa agaatgatcc
      181 aagcccggct ttccctctgt gcgcggattg ttattttttt agacttattt ctatgtttgt
      241 tttggaccta atataactta gtcatgaatc ctggttgact cgcgcgcatc tttttccact
      301 ctgcgatgat tcacagtgca gcgtgcagct cagctccagc agcagtacca gcggcagctc
      361 caacagtgga gccttccttt cacgaacgga atagaaacag gaaggaaaaa aatagaccaa
      421 caagaggacc catccgcgac ttctaatttt tggcgcatga accgcgctgt tctacccgcc
      481 cgtccgtcct catccctccc ccccaggaaa acaggaaatt gggaaaccta ggtgcgtgca
      541 ttttcgtttt gggcgatgat tgacaatgag acttagtccg cttggttggg tttcgtttca
      601 aaaacaaggt ttggaagaag atcttgggag agaaagaccg agagatcttg accagggcga
      661 agacgaccag tagcttttgg tatagccggg ataagcttca ggcaccctag ggttgtataa
      721 gagttgtatg caaggatgtg cgcaagggtt tctgaagcag gatggaaatg tggtgtgttt
      781 ttttgtagag caattgcctt aaagtcgggg attacgccga gacagcagct tgacggttcc
      841 gtggtaatat gtgtccggca acttggcgcg cctcagccga tgagaccaga ccaataaaaa
      901 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      961 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     1021 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1081 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1141 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1201 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     1261 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1321 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1381 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1441 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1501 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1561 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1621 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1681 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1741 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     1801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1981 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2461 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2521 taaccgtag
//
