LOCUS       sequence-244914-        2660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P19
FEATURES             Location/Qualifiers
     source          1..2660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1025..1059)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1130..1789)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1704..1723
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1971..2559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2460..2479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgagccatc caggccaggt agaaatagag aaagccgaat gttagacaat
       61 atggcagcgt agtagagtag gtaggtaggc aagtactgct agcaaagagg agaagggtaa
      121 gctcactctt cgcattccac accgttagtg tgtcagtttg aacaaaaaaa caatcatcat
      181 accaattgat ggactgtgga ctggcttttg gaacggcttt tcggactgcg attattcgtg
      241 aggaatcaag gtaggaattt ggtcatattt acggacaaca gtgggtgatt cccatatgga
      301 gtaggaaaac gagatcatgg tatcctcaga tatgttgcgg aattctgttc accgcaaagt
      361 tcagggtgct ctggtgggtt tcggttggtc tttgctttgc ttctcccttg tcttgcatgt
      421 taataatagc ctagcctgtg agccgaaact tagggtaggc ttagtgttgg aacgtacata
      481 tgtatcacgt tgacttggtt taaccaggcg acctggtagc cagccatacc cacacacgtt
      541 ttttgtatct tcagtatagt tgtgaaaagt gtagcggaaa tttgtggtcc gagcaacagc
      601 gtctttttct agtagtgcgg tcggttactt ggttgacatt ggtatttgga ctttgttgct
      661 acaccattca ctacttgaag tcgagtgtga agggtatgat ttctagtggt gaacaccttt
      721 agttacgtaa tgttttcatt gctgttttac ttgagatttc gattgagaaa aaggtattta
      781 atagctcgaa tcaatgtgtt atcattgtga agatgttctt ccctaactcg aaaggtatat
      841 gaggcttgtg tttcttagga gaattattat tcttttgtta tgttgcgctt gtagttggaa
      901 aaggtgaaga gacaaaagcg cttaacactt gaaatttagg aaagagcaga atttggcaaa
      961 aaaaataaaa aaaaaataaa cacacatact catcgagaac tgaaagatat gtgagaccag
     1021 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1081 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1141 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1201 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1261 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1321 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
     1381 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1441 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1501 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1561 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1621 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1681 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1741 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1801 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     1861 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     1921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2101 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2641 gattctgtgg ataaccgtag
//
