LOCUS       sequence-244916-        2664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P2
FEATURES             Location/Qualifiers
     source          1..2664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1029..1063)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1134..1793)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1708..1727
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1975..2563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2464..2483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtccagcg aatatacagc gtgaataatg gaatggcctt gtattcgttt
       61 tttccgagag aaaaaaacgg gcttcggtga aaatcgggtg aatatgcaac tagcgggacg
      121 aatgctctgg aaatgcatat cctatgcaac tagcgggatg aacaaatctc accccagaat
      181 tcgcaggaaa aaacaggaaa aaaaaaaaga aggccaccac ggccacaaag accacaaaga
      241 ccacaaaaaa aaacaaaaaa caaccgtccc agcttccagt gtttggaata ctggaacaca
      301 ggaagccgca taagagtggg cgttgcacag gaagccaggc ccagaagccc cagagttact
      361 tttttttttt tgttttttcc ttctgttcgc tgtgcccgca tcagatgatg cgcctttatt
      421 tacgatgcca atgcgaatag caccagtgag agcaccagta aaagcatacg catacacata
      481 cacacataga gcaagcaagc aggctagcaa ccaggaaagg ctgccagtga ctgctactgg
      541 gtgtctaaga accgtagggc ggattattgt tgcggtggtt ggttgcgggt ggttatgcga
      601 tggtacggtg cagaatcgta cggtgttggt tatggaatta gtatgggtat gtgatatgtg
      661 gtaatatgtg atattgggtt attgtgattt ggaatactga atatcgaata tgggatatgg
      721 aatatggcca tggcatggta tggtatggga tgggagtatt ctattttatt ttattttatt
      781 ctggttcctg cgtttagggt agggtaggaa gaaggtgagt gcttttgtat ataagtggag
      841 tgtctggatc agttttgtgg attgtgaatg ttagtttccc ctttaatgta tatttgtatt
      901 atttgctttt gagtactcaa taaccaagca caactactag ttttaaagga tccatcctct
      961 taaacagtac aaatcgcaaa gaaaagctcc acacccaaac caaataattg ctatgtgaga
     1021 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1081 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1141 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1201 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1261 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1321 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     1381 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1441 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1501 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1561 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     1621 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     1681 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     1741 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     1801 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     1861 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
     1921 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1981 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2041 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2101 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2161 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2221 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2281 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2341 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2401 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2461 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2521 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2581 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2641 ccctgattct gtggataacc gtag
//
