LOCUS       sequence-244918-       10350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector for transient TYLCV V2 and fLUC (with intron) 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB117 - pL2_pSB90_fLUC-I_V2
FEATURES             Location/Qualifiers
     source          1..10350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        445..790
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1244..1496
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        1946..2291
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2448..2466)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2526..2714
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      4236..4488
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4554..4578
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4858..5487
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5916..6989
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7055..7249
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        7311..7415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7416..8210
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9535..10123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10024..10043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10259..10283
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gtcaacatgg tggagcacga
       61 cactctggtc tactccaaaa atgtcaaaga tacagtctca gaagatcaaa gggctattga
      121 gacttttcaa caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
      421 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
      481 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
      661 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac
      841 aacaaacaac attacaatta catttacaat tatcgataca atgtgggacc cacttctaaa
      901 tgaatttcct gaatctgttc acggatttcg ttgtatgtta gctattaaat atttgcagtc
      961 cgttgaggaa acttacgagc ccaatacatt gggccacgat ttaattaggg atcttatatc
     1021 tgttgtaagg gcccgtgact atgtcgaagc gaccaggcga tataatcatt tccacgccag
     1081 actcgaaggt tcgccgaagg ctgaacttcg acagcccata cagcagccgt gctgctgtcc
     1141 ccattgtcca aggcacaaac aagcgacgat catggacgta caggcccatg taccgaaagc
     1201 ccagaatata cagaatgtat cgaagccctg agcttgtcaa gcagatcgtt caaacatttg
     1261 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     1321 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     1381 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     1441 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg
     1501 ctactagcaa catacgctgg attacgaatt cccatgggga ggtcaacatg gtggagcacg
     1561 acactctggt ctactccaaa aatgtcaaag atacagtctc agaagatcaa agggctattg
     1621 agacttttca acaaaggata atttcgggaa acctcctcgg attccattgc ccagctatct
     1681 gtcacttcat cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg
     1741 ataaaggaaa ggctatcatt caagatctct ctgccgacag tggtcccaaa gatggacccc
     1801 cacccacgag gagcatcgtg gaaaaagaag aggttccaac cacgtctaca aagcaagtgg
     1861 attgatgtga taacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     1921 cagtctcaga agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     1981 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2041 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2101 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg
     2161 ttccaaccac gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2221 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2281 tggagaggac acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa
     2341 caacaaacaa cattacaatt acatttacaa ttatcgatac aatggaggac gccaaaaaca
     2401 taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc
     2461 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     2521 tcgaggtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag
     2581 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt
     2641 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat
     2701 ttgttgatgt gcaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     2761 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     2821 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     2881 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     2941 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3001 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     3061 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     3121 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     3181 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     3241 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     3301 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     3361 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     3421 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     3481 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     3541 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     3601 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     3661 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     3721 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     3781 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     3841 cttactggga cgaggacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     3901 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     3961 tcttcgacgc gggcgtggca ggtctgcccg acgatgacgc cggtgaactt cccgccgccg
     4021 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     4081 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     4141 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     4201 agggcggaaa gtccaaattg taagcttgtc aagcagatcg ttcaaacatt tggcaataaa
     4261 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     4321 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     4381 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     4441 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga cgctcagaga
     4501 ggatgcacat gtgaccgagg gacacgaagt gatccgttta aactatcagt gtttgacagg
     4561 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     4621 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg ccagccgtgc ggctgcatga
     4681 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     4741 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     4801 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     4861 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     4921 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     4981 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     5041 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     5101 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     5161 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     5221 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     5281 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     5341 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     5401 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     5461 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     5521 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     5581 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     5641 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     5701 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     5761 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     5821 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
     5881 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
     5941 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     6001 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     6061 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgtcg tcgattagga
     6121 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     6181 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     6241 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     6301 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     6361 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     6421 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     6481 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     6541 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     6601 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     6661 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     6721 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     6781 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     6841 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     6901 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     6961 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     7021 tagcagggga aaaaggtcga aaaagcttct ttcctgtgga tagcacgtac attgggaacc
     7081 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     7141 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     7201 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     7261 agcgcacagc cgaacagctg caaaaagcgc ccacatcaag gctccgagtg cgcggaaccc
     7321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7381 gataaatgct tcaataatat tgaaaaagga agagtatggc taaaatgaga atatcaccgg
     7441 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     7501 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     7561 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     7621 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     7681 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     7741 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     7801 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
     7861 acgatctggc cgatgtggat tgcgaaaact gggaagagga cactccattt aaagatccgc
     7921 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     7981 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     8041 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgctca
     8101 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     8161 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag ctgtcagacc
     8221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttct
     8341 agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt cataaaattg
     8401 aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg aactgcccat
     8461 ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg tgaacaaggg
     8521 ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg atgacgacgt
     8581 cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag tgaccgcggt
     8641 agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg tcgtggttgt
     8701 tgatctagat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg cggcaaagtc
     8761 tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata aagttgttgc
     8821 actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag agtttctagc
     8881 acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag accgacggtc
     8941 tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt ccgaagctgg
     9001 cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt tagagaaacc
     9061 ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg acgaggaggt
     9121 ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg aggcggatcc
     9181 gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg acgcggacgt
     9241 gcaggtttcg cacgggggga cgatggcagc ctgagccaat tgcatttgcc tcttaattat
     9301 ctggctcaaa gggtgactga ggagtaagcg atgtgcccat cacactgacc accaagacgg
     9361 gggttcgttt tgaagatcac ccttcagacc caaggccagt gtgcgttgat cgcggccaca
     9421 ctgcaaattc acatactgtc gataaagggg cgttggacga gaacccattc gtcaggatac
     9481 tggctcgtga tctcactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9541 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9601 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9661 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9721 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9781 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9841 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9901 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9961 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10021 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10081 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10141 ctttttacgg ttcctgctcg gatctgttgg accggacagt agtcatggtt gatgggctgc
    10201 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    10261 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    10321 cgtttttaat gtactggggt tgaacactct
//
