LOCUS       sequence-244921-       10985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression empty vector under CAG promoter with EGFP 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, 
            Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen 
            P
  TITLE     Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through 
            Delta/Notch Regulation.
  JOURNAL   Cell. 2018 May 31;173(6):1370-1384.e16. doi: 
            10.1016/j.cell.2018.03.067. Epub 2018 May 31.
  PUBMED    29856955
REFERENCE   2  (bases 1 to 10985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.03.067"; journalName: "Cell"; date: 
            "2018-05-31- 31"; volume: "173"; issue: "6"; pages: "1370-1384.e16"
COMMENT     Sequence Label: pLenti-CAG-empty-ires-EGFP
FEATURES             Location/Qualifiers
     source          1..10985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        109..412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     gap             558..574
                     /estimated_length=17
     intron          691..1707
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1631..1653
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1715..1734
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1770..1789
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1770..1788
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1803..1821
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1803..1820
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(1854..2428,2429..2431,2432..2440)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2021..2038)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2248..2267
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2441..2443,2444..2446,2447..3160)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2441..2443,2444..2446,2447..3157)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2486..2507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2747..2766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3094..3115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3184..3772
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3237..3257)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3655..3666)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3775..3791)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3776..3792)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4297..4477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4503..4520)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4509..4733
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4779..5207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4866..4885)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5076..5097
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5216..5236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5221..5550
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5401..5536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5463..5482
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5598..5645
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5664..6038
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6168..6289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6205..6224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6259..6278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6338..6354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6338..6354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6351..6373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6362..6378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6386..6392,6393..6410,6411..6416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6431..6452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6569..6586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6740..7328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6820..6839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7499..8290,8291..8359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8122..8141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8360..8464)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8539..8558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8730..9109
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9111..9309
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9264..9284
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9327..9507
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9554..9679
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10172..10405
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10590..10634
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(10932..10985,1..64)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
       61 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaatccgcg ttacataact
      121 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      181 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      241 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      301 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      361 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
      421 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
      481 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg ggggcgcgcg
      541 ccaggsgggg cggggssnnn nnnnnnnnnn nnnngggsga ggcggagagg tgcggcggca
      601 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
      661 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
      721 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
      901 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
      961 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
     1081 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     1141 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     1201 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     1261 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg
     1321 gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     1381 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     1441 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     1501 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     1561 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     1621 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     1681 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1741 catcattttg gcaaagaatt gctcgagcgt aatacgactc actataggga gacccaagct
     1801 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccgcccctct
     1861 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     1921 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     1981 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     2041 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     2101 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     2161 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     2221 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     2281 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc
     2341 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     2401 gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga
     2461 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2521 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2581 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2641 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2701 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2761 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2821 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2881 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2941 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3001 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     3061 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3121 cgccgggatc actctcggca tggacgagct gtacaagtaa aattcgatat caagcttatc
     3181 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3241 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3301 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3361 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3421 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3481 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3541 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3601 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3661 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3721 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3781 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     3841 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     3901 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3961 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     4021 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     4081 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     4141 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     4201 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     4261 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     4321 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4381 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4441 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     4501 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4561 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4621 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4681 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4741 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4801 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4861 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4921 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4981 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5041 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5101 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5161 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5221 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5281 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5341 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5461 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5521 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5581 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     5641 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     5701 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     5761 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     5821 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     5881 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     5941 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     6001 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     6061 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6121 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6301 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6541 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6601 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6661 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6721 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6781 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6841 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6901 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6961 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7021 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7081 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7141 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7201 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7261 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7321 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7381 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7441 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7501 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7561 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7621 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7681 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7741 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7801 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7861 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7921 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7981 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8041 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8101 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8161 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8221 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8281 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8341 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8401 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8461 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8521 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8581 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8641 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8701 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     8761 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8821 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     8881 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8941 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     9001 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     9061 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     9121 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     9181 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     9241 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     9301 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9361 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9421 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9481 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     9541 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9601 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9661 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9721 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9781 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9841 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9901 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9961 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
    10021 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    10081 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    10141 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    10201 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    10261 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10321 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10381 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10441 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10501 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10561 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10621 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10681 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10741 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10801 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    10861 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
    10921 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10981 gtaga
//
