LOCUS       sequence-24493-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J
  TITLE     TGFbeta primes breast tumors for lung metastasis seeding through 
            angiopoietin-like 4.
  JOURNAL   Cell. 2008 Apr 4. 133(1):66-77.
  PUBMED    18394990
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Apr 4. 133(1):66-77."
COMMENT     Sequence Label: pRetroSuper shAngptl4 puro
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..32
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     17..33
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(106..320)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(166..185)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(366..389)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     762..781
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ctcgatgatg atctctcgag gtcgacggta tcgataagct tttccaaaaa cagcaggatc
       61 cagcaactct ctcttgaaga gttgctggat cctgctgggg gatctgtggt ctcatacaga
      121 acttataaga ttcccaaatc caaagacatt tcacgtttat ggtgatttcc cagaacacat
      181 agcgacatgc aaatattgca gggcgccact cccctgtccc tcacagccat cttcctgcca
      241 gggcgcacgc gcgctgggtg ttcccgccta gtgacactgg gcccgcgatt ccttggagcg
      301 ggttgatgac gtcagcgttc gaattctacc gggtagggga ggcgcttttc ccaaggcagt
      361 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
      421 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
      481 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
      541 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
      601 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
      661 tcgctttctg ggctcagagg ctgggaggnn nntgggtccg ggggcgggct cagggggggc
      721 tcagtggcgg ggcgggcgcc cgaaggtcct ccggagnccg gcattctgca cgcttcaaaa
      781 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg gg
//
