LOCUS       sequence-244943-        2399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA 
            binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: p5xNF-kb_RE
FEATURES             Location/Qualifiers
     source          1..2399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(79..97)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(80..97)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(113..129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(113..129)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(244..832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(324..343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        947..1049
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1050..1709
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1116..1135)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1822..2277
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1909..1928)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2119..2140
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2296..2313
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2297..2313
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2320..2339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2320..2338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agtccccgga aattcccgga aagtccccgg aaattcccct cccgagacca ctcatcgccc
       61 aatcactagt gaattcgttc tatagtgtca cctaaatacc atagagccgt tggtcatagc
      121 tgtttcctgt ccaggcatca tgacacggtt atccacagaa tcaggggata acgcaggaaa
      181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      661 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgtgatgat cggcacgtaa
      961 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
     1021 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     1081 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     1141 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     1201 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     1261 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     1321 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     1381 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1441 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtgtcagcc aatccctggg
     1501 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1561 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1621 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1681 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     1741 cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgatga
     1801 taagctgtca aacatgatgt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1861 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1921 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1981 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2041 gtgatggttc acgtagtggg ccagcgccct gatagacggt ttttcgccct ttgacgttgg
     2101 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     2161 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     2221 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaattttaa
     2281 cattgtagaa acgcatgtaa aacgacggcc agtgaattgt aatacgactc actatagggt
     2341 gaattccgct taggacctta gatattgatc tcaaggtctc ggggaggaaa ttcccggaa
//
