LOCUS       sequence-244944-        4027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - ELuc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pELuc
FEATURES             Location/Qualifiers
     source          1..4027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..65
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     49..65
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     72..91
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1858..1876)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1859..1876)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1892..1908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1892..1908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(2023..2611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2103..2122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2726..2828
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2829..3488
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2895..2914)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(3601..4027,1..2)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3688..3707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3898..3919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttaacaaa atattaacgc ttacaatttt aacattgtag aaacgcatgt aaaacgacgg
       61 ccagtgaatt gtaatacgac tcactatagg gtgaattccg cttaggacct tagatattga
      121 tctcaaggtc tcgaagctca agccgccagg gcatagttct catcgttggc ggcggatgtc
      181 ttgtacatag atccaccacc cgccttctcc ataagtgctt ctcggagttc ctttcggctg
      241 attttgcccg tgacgttcct cggaatagag tcaacgaatc tgacaccccc tcgaagatac
      301 ttgctgtggc taactctttg tgcaaggaaa tcatatactt ctttagccgt cagttgggcc
      361 ccaggttgct tgacaacgaa tccggccgga agttcccctg cttctatgtc ggggataccg
      421 actacggcca catcgcggat ccctggatgt tggagcaaaa tctcctccaa ctcgacagga
      481 gccacttgat atcccttgta cttgatcagc tctttatatc tatccacaat gtaaaaatat
      541 tcatcctcat catagtagcc aaaatcacca ctgtgaagcc aaccatcgtc gtcaatggcc
      601 tccttggtgg cctgtggatt attcacgtag cctttcgtga ccatggggcc ccaaatgcac
      661 aactctccca cttgatttgg gccaagcgcc tctccggtgt ttcgatctat aattttagcg
      721 gccatgtagg gcgtcacttt tcccaatgaa ccggatttaa attcgttgtg aagagtgtgg
      781 atattagcgg atgtgctttc agtcaacccg taaccacacc gtatgcctgg caaattaagg
      841 cgttttacgg cgatttcagc tacttctttt gcaagaggtg ccgcaccaca acaaagttcc
      901 gccaaggtag aaagatcata cttatcaacc aacggtgatt tagaaagaaa gaggattgta
      961 cttgggacat tgatcacact tcgtacttca tagtcttgaa tcgccttcaa aaaaacctct
     1021 tggttgaacc tccgaagcat cacgacgcgc agccccacca taaaataccc caagttaatg
     1081 gaaaacccaa aggcgtgaaa gaagggaaga tacgccagca cactaactcc gggaatgagt
     1141 tgggtaccaa ctctcgggtc agaggcgtgg gtcagccgaa cacatatatt gcggtgggtc
     1201 tgcatgacgc ccttaggcaa cccggtagta ccactactac acagtatcgc tgcaacctgg
     1261 tcgaccggat cgtaatgcag tggtttaaag gtctgaagat tgttatcgct atatcttgac
     1321 ataaaattat gcaaggattc acatccgaga aggttttcct cagagtccag aattatgatt
     1381 cttttgatat aatttgttct atccttaacc tccaaaacct ttggaagtat ctttcgcgtg
     1441 gtgaacacaa gtattggctt actaattcct gtgaccttac acagttcgtc tggtatatag
     1501 tcctcgttta caggtgcaac gaccatgcct atataccacg cactaataat tggaatgaaa
     1561 aatcttttgt tgttttcagc gcatattgat acaacatcat tcattttata gccacaattc
     1621 tggagactct gccccaattt gaccgtcgtg tcgaagaatt cctgataact aagactttcg
     1681 ttacccatca cgtcgaggat cgcctgcggg atgtgactat gtttgtgaag cgcgttgtag
     1741 agcatctctc cagcggtaaa attcccaagc gggtgcttag gctcgggccc atacacaaca
     1801 ttcttctctc tcttcatcat tcgagaccac tcatcgccca atcactagtg aattcgttct
     1861 atagtgtcac ctaaatacca tagagccgtt ggtcatagct gtttcctgtc caggcatcat
     1921 gacacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2461 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2581 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2641 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2701 gatcttcacc tagatccttt tgtgatgatc ggcacgtaag aggttccaac tttcaccata
     2761 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     2821 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     2881 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     2941 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     3001 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     3061 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     3121 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     3181 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     3241 ttattgagaa tatgtttttc gtgtcagcca atccctgggt gagtttcacc agttttgatt
     3301 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     3361 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     3421 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     3481 gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc tacgcctgaa
     3541 taagtgataa taagcggatg aatggcagaa attcgatgat aagctgtcaa acatgatgtt
     3601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3841 cagcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4021 acgcgaa
//
