LOCUS       sequence-244956-        7275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the VP2del30 truncation mutant protein derived from AAV2 
            VP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Thadani NN, Dempsey C, Zhao J, Vasquez SM, Suh J
  TITLE     Reprogramming the Activatable Peptide Display Function of 
            Adeno-Associated Virus Nanoparticles.
  JOURNAL   ACS Nano. 2018 Feb 27;12(2):1445-1454. doi: 10.1021/acsnano.7b07804.
            Epub 2017 Dec 26.
  PUBMED    29278489
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.7b07804"; journalName: "ACS Nano"; date: 
            "2018-02-27- 27"; volume: "12"; issue: "2"; pages: "1445-1454"
COMMENT     Sequence Label: pVP2del30
FEATURES             Location/Qualifiers
     source          1..7275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     104..126
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     118..135
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     119..135
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     145..164
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        145..163
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     188..204
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     189..205
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(239..255)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(239..255)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(486..497)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(2043..2060)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4605..4625)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4605..4623)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4644..4660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4644..4660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4657..4679)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4668..4684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4692..4698,4699..4716,4717..4722))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4737..4758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4875..4892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5046..5634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5126..5145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5805..6596,6597..6665))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6428..6447
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6666..6770)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6797..7253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6884..6903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7094..7115
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
       61 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      121 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
      181 ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
      241 tccactagtt ctagagcatg gaaactagat aagaaagaaa tacgcagaga ccaaagttca
      301 actgaaacga attaaacggt ttattgatta acaagcaatt acagattacg agtcaggtat
      361 ctggtgccaa tggggcgagg ctctgaatac acgccattag tgtccacagt aaagtccaca
      421 ttaacagact tgttgtagtt ggaagtgtac tgaatttcgg gattccagcg tttgctgttt
      481 tccttctgca gctcccactc gatctccacg ctgacctgtc ccgtggagta ctgtgtgatg
      541 aaggaagcaa actttgccgc actgaaggtg gtcgaaggat tcgcaggtac cggggtgttc
      601 ttgatgagaa tctgtggagg agggtgttta agtccgaatc cacccatgag gggagagggg
      661 tgaaaatgtc cgtccgtgtg tggaatcttt gcccagatgg gcccctgaag gtacacatct
      721 ctgtcctgcc agaccatgcc tggaagaacg ccttgtgtgt tgacatctgc ggtagctgct
      781 tgtctgttgc ctctctggag gttggtagat acagaaccat actgctccgt agccacggga
      841 ttggttgtcc tgatttcctc ttcgtctgta atcatgacct tttcaatgtc cacatttgtt
      901 ttctctgagc cttgcttccc aaagatgaga accccgctct gaggaaaaaa cttttcttca
      961 tcgtccttgt ggcttgccat ggccgggccc ggattcacca gagagtctct gccattgagg
     1021 tggtacttgg tagctccagt ccacgagtat tcactgttgt tgttatccgc agatgtcttt
     1081 gatactcgct gctggcggta acagggtcca ggaagccagt tcctagactg gtcccgaatg
     1141 tcactcgctc cggcctgaga aaactgaagc cttgactgcg tggtggttcc acttggagtg
     1201 tttgttctgc tcaagtaata caggtactgg tcgatgagag gattcatgag acggtccaga
     1261 ctctggctgt gagcgtagct gctgtggaaa ggaacgtcct caaaagtgta gctgaaggta
     1321 aagttgtttc cggtacgcag catctgagaa ggaaagtact ccaggcagta aaatgaagag
     1381 cgtcctactg cctgactccc gttgttcagg gtgaggtatc catactgtgg caccatgaag
     1441 acgtctgctg ggaacggcgg gaggcatcct tgatgcgccg agccgaggac gtacgggagc
     1501 tggtactccg agtcagtaaa cacctgaacc gtgctggtaa ggttattggc aatcgtcgtc
     1561 gtaccgtcat tctgcgtgac ctctttgact tgaatgttaa agagcttgaa gttgagtctc
     1621 ttgggtcgga atccccagtt gttgttgatg agtctttgcc agtcacgtgg tgaaaagtgg
     1681 cagtggaatc tgttgaagtc aaaatacccc caaggggtgc tgtagccaaa gtagtgattg
     1741 tcgttcgagg ctcctgattg gctggaaatt tgtttgtaga ggtggttgtt gtaggtgggc
     1801 agggcccagg ttcgggtgct ggtggtgatg actctgtcgc ccagccatgt ggaatcgcaa
     1861 tgccaatttc ccgaggaatt acccactccg tcggcgccct cgttattgtc tgccagtggt
     1921 gcgccactgc ctgtagccag cgtattagtt cccagaccag agggggctgc tggtggctgt
     1981 ccgagaggct gggggtcagg tactgagtct gcgtctccag tctgaccaaa attcaatctt
     2041 ttgtggtggt ggtggtggtg cattcttgca ggctgctggc ccgcctttcc ggttcccgag
     2101 gaggagtctg gctccacagg agagtgctct accggcctct tttttcccgg agccgcctta
     2161 acaggttcct caaccaggcc cagaggttca agaaccctct ttttcgcctg gaagactgct
     2221 cgtccgaggt tgcccccaaa agacgtatct tctttaaggc gctcctgaaa ctccgcgtcg
     2281 gcgtggttgt acttgaggta cgggttgtct ccgctgtcga gctgccggtc gtaggctttg
     2341 tcgtgctcga gggccgcggc gtctgcctcg ttgaccggct ctcccttgtc gagtccgttg
     2401 aagggtccga ggtacttgta cccaggaagc acaagacccc tgctgtcgtc cttatgccgc
     2461 tctgcgggct ttggtggtgg tgggccaggt ttgagcttcc accactgtct tattccttca
     2521 gagagagtgt cctcgagcca atctggaaga taaccatcgg cagccagacc tgatttaaat
     2581 catttattgt tcaaagatgc agtcatccaa atccacattg accagatcgc aggcagtgca
     2641 agcgtctggc acctttccca tgatatgatg aatgtagcac agtttctgat acgccttttt
     2701 gacgacagaa acgggttgag attctgacac gggaaagcac tctaaacagt ctttctgtcc
     2761 gtgagtgaag cagatatttg aattctgatt cattctctcg cattgtctgc agggaaacag
     2821 catcagattc atgcccacgt gacgagaaca tttgttttgg tacctgtctg cgtagttgat
     2881 cgaagcttcc gcgtctgacg tcgatggctg cgcaactgac tcgcgcaccc gtttgggctc
     2941 acttatatct gcgtcactgg gggcgggtct tttcttggct ccaccctttt tgacgtagaa
     3001 ttcatgctcc acctcaacca cgtgatcctt tgcccaccgg aaaaagtctt tgacttcctg
     3061 cttggtgacc ttcccaaagt catgatccag acggcgggtg agttcaaatt tgaacatccg
     3121 gtcttgcaac ggctgctggt gttcgaaggt cgttgagttc ccgtcaatca cggcgcacat
     3181 gttggtgttg gaggtgacga tcacgggagt cgggtctatc tgggccgagg acttgcattt
     3241 ctggtccacg cgcaccttgc ttcctccgag aatggctttg gccgactcca cgaccttggc
     3301 ggtcatcttc ccctcctccc accagatcac catcttgtcg acacagtcgt tgaagggaaa
     3361 gttctcattg gtccagttta cgcacccgta gaagggcaca gtgtgggcta tggcctccgc
     3421 gatgttggtc ttcccggtag ttgcaggccc aaacagccag atggtgttcc tcttgccgaa
     3481 ctttttcgtg gcccatccca gaaagacgga agccgcatat tggggatcgt acccgtttag
     3541 ttccaaaatt ttataaatcc gattgctgga aatgtcctcc acgggctgct ggcccaccag
     3601 gtagtcgggg gcggttttag tcaggctcat aatctttccc gcattgtcca aggcagcctt
     3661 gatttgggac cgcgagttgg aggccgcatt gaaggagatg tatgaggcct ggtcctcctg
     3721 gatccactgc ttctccgagg taatcccctt gtccacgagc cacccgacca gctccatgta
     3781 cctggctgaa gtttttgatc tgatcaccgg cgcatcagaa ttgggattct gattctcttt
     3841 gttctgctcc tgcgtctgcg acacgtgcgt cagatgctgc gccaccaacc gtttacgctc
     3901 cgtgagattc aaacaggcgc ttaaatactg ttccatatta gtccacgccc actggagctc
     3961 aggctgggtt ttggggagca agtaattggg gatgtagcac tcatccacca ccttgttccc
     4021 gcctccggcg ccatttctgg tctttgtgac cgcgaaccag tttggcaaag tcggctcgat
     4081 cccgcggtaa attctctgaa tcagtttttc gcgaatctga ctcaggaaac gtcccaaaac
     4141 catggatttc accccggtgg tttccacgag cacgtgcatg tggaagtagc tctctccctt
     4201 ctcaaattgc acaaagaaaa gggcctccgg ggccttactc acacggcgcc attccgtcag
     4261 aaagtcgcgc tgcagcttct cggccacggt caggggtgcc tgctcaatca gattcagatc
     4321 catgtcagaa tctggcggca actcccattc cttctcggcc acccagttca caaagctgtc
     4381 agaaatgccg ggcagatgct cgtcaaggtc gctggggacc ttaatcacaa tctcgtaaaa
     4441 ccccggcatg gcggctgcgc gttcaaacct cccgcttcaa aatggagacc ctgcgtgctc
     4501 actcgggctt aaatacccag cgtgaccaca tggtgtcgca aaatgtcgca aaacactcac
     4561 gtgacctcta atacaggacc tctagagcgg cccagctttt gttcccttta gtgagggtta
     4621 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4801 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5101 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5161 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5221 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5281 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5341 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5401 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5461 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5521 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5581 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5641 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5701 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5761 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5821 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5881 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5941 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6001 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6061 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6121 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6181 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6241 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6301 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6361 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6421 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6481 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6541 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6601 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6661 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6721 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6781 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6841 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6901 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6961 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7021 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7081 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7141 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct
     7201 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg
     7261 ccattcaggc tgcgc
//
