LOCUS       sequence-24496-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J
  TITLE     TGFbeta primes breast tumors for lung metastasis seeding through 
            angiopoietin-like 4.
  JOURNAL   Cell. 2008 Apr 4. 133(1):66-77.
  PUBMED    18394990
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Apr 4. 133(1):66-77."
COMMENT     Sequence Label: pRetroSuper shSmad4J puro
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(111..127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(146..562)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(162..178)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(194..216)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tttggatttg ggatcttata agttctgtat gagaccacag atccccggtg tgcagttgga
       61 atgtattcaa gagatacatt ccaactgcac acctttttgg aaaagcttat cgataccgtc
      121 gacctcgaga gatcgatcta attccggcgc ctagagaagg agtgagggct ggataaaggg
      181 aggatcgagg cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa
      241 gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca
      301 aggcttccca ggtcacgatg taggggacct ggtctgggtg tccatgcggg ccaggtgaaa
      361 agaccttgat cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc
      421 cgacgttaaa ggttggccat tctgcagagc agaaggtaac ccaacgtctc ttcttgacat
      481 ctaccgactg gttgtgagcg atccgctcga catctttcca gtgacctaag gtcaaactta
      541 agggagtggt aacagtctgg ccctaatttt cagacaaata cagaaacaca gtcagacaga
      601 gacaacacag aacgatgctg cagcgctgca gcagacaaga cgcgcggctt cggttccaaa
      661 ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa
      721 ccacatatcc tgacggggtc ggattccaca tcgactccct tcctcaggtc gggccacaaa
      781 aaacggcccc caaagtccct gggacgtctc ccagggttgc ggccgggntg ttcagaanct
      841 cgtcagttcc accacgggtt ccgccagata cagagctagt tagctaacta gtacanacnc
      901 aggcgcaaac attaaatgcc gggcncaaac ccccccaaag acngagacng acccgaaacg
      961 accgctggnc cagcttacct ccccgggggg ggggtcggtg
//
