LOCUS       sequence-244962-        7275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the VP2del50 truncation mutant protein derived from AAV2 
            VP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Thadani NN, Dempsey C, Zhao J, Vasquez SM, Suh J
  TITLE     Reprogramming the Activatable Peptide Display Function of 
            Adeno-Associated Virus Nanoparticles.
  JOURNAL   ACS Nano. 2018 Feb 27;12(2):1445-1454. doi: 10.1021/acsnano.7b07804.
            Epub 2017 Dec 26.
  PUBMED    29278489
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.7b07804"; journalName: "ACS Nano"; date: 
            "2018-02-27- 27"; volume: "12"; issue: "2"; pages: "1445-1454"
COMMENT     Sequence Label: pVP2del50
FEATURES             Location/Qualifiers
     source          1..7275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..98
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     332..349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    466..487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(502..507,508..525,526..532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    540..556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     545..567
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     564..580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     564..580
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     599..619
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        601..619
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3224..3241
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4727..4738
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     4969..4985
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4969..4985
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5019..5035)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5020..5036)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5060..5079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5061..5079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5089..5106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5089..5105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5098..5120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5246..5702)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5384..5405)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5596..5615
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5729..5833
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5834..5902,5903..6694)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6052..6071)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6865..7275,1..178)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
       61 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      121 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      181 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      241 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      301 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      361 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      421 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      481 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      541 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      601 aattaaccct cactaaaggg aacaaaagct gggccgctct agaggtcctg tattagaggt
      661 cacgtgagtg ttttgcgaca ttttgcgaca ccatgtggtc acgctgggta tttaagcccg
      721 agtgagcacg cagggtctcc attttgaagc gggaggtttg aacgcgcagc cgccatgccg
      781 gggttttacg agattgtgat taaggtcccc agcgaccttg acgagcatct gcccggcatt
      841 tctgacagct ttgtgaactg ggtggccgag aaggaatggg agttgccgcc agattctgac
      901 atggatctga atctgattga gcaggcaccc ctgaccgtgg ccgagaagct gcagcgcgac
      961 tttctgacgg aatggcgccg tgtgagtaag gccccggagg cccttttctt tgtgcaattt
     1021 gagaagggag agagctactt ccacatgcac gtgctcgtgg aaaccaccgg ggtgaaatcc
     1081 atggttttgg gacgtttcct gagtcagatt cgcgaaaaac tgattcagag aatttaccgc
     1141 gggatcgagc cgactttgcc aaactggttc gcggtcacaa agaccagaaa tggcgccgga
     1201 ggcgggaaca aggtggtgga tgagtgctac atccccaatt acttgctccc caaaacccag
     1261 cctgagctcc agtgggcgtg gactaatatg gaacagtatt taagcgcctg tttgaatctc
     1321 acggagcgta aacggttggt ggcgcagcat ctgacgcacg tgtcgcagac gcaggagcag
     1381 aacaaagaga atcagaatcc caattctgat gcgccggtga tcagatcaaa aacttcagcc
     1441 aggtacatgg agctggtcgg gtggctcgtg gacaagggga ttacctcgga gaagcagtgg
     1501 atccaggagg accaggcctc atacatctcc ttcaatgcgg cctccaactc gcggtcccaa
     1561 atcaaggctg ccttggacaa tgcgggaaag attatgagcc tgactaaaac cgcccccgac
     1621 tacctggtgg gccagcagcc cgtggaggac atttccagca atcggattta taaaattttg
     1681 gaactaaacg ggtacgatcc ccaatatgcg gcttccgtct ttctgggatg ggccacgaaa
     1741 aagttcggca agaggaacac catctggctg tttgggcctg caactaccgg gaagaccaac
     1801 atcgcggagg ccatagccca cactgtgccc ttctacgggt gcgtaaactg gaccaatgag
     1861 aactttccct tcaacgactg tgtcgacaag atggtgatct ggtgggagga ggggaagatg
     1921 accgccaagg tcgtggagtc ggccaaagcc attctcggag gaagcaaggt gcgcgtggac
     1981 cagaaatgca agtcctcggc ccagatagac ccgactcccg tgatcgtcac ctccaacacc
     2041 aacatgtgcg ccgtgattga cgggaactca acgaccttcg aacaccagca gccgttgcaa
     2101 gaccggatgt tcaaatttga actcacccgc cgtctggatc atgactttgg gaaggtcacc
     2161 aagcaggaag tcaaagactt tttccggtgg gcaaaggatc acgtggttga ggtggagcat
     2221 gaattctacg tcaaaaaggg tggagccaag aaaagacccg cccccagtga cgcagatata
     2281 agtgagccca aacgggtgcg cgagtcagtt gcgcagccat cgacgtcaga cgcggaagct
     2341 tcgatcaact acgcagacag gtaccaaaac aaatgttctc gtcacgtggg catgaatctg
     2401 atgctgtttc cctgcagaca atgcgagaga atgaatcaga attcaaatat ctgcttcact
     2461 cacggacaga aagactgttt agagtgcttt cccgtgtcag aatctcaacc cgtttctgtc
     2521 gtcaaaaagg cgtatcagaa actgtgctac attcatcata tcatgggaaa ggtgccagac
     2581 gcttgcactg cctgcgatct ggtcaatgtg gatttggatg actgcatctt tgaacaataa
     2641 atgatttaaa tcaggtctgg ctgccgatgg ttatcttcca gattggctcg aggacactct
     2701 ctctgaagga ataagacagt ggtggaagct caaacctggc ccaccaccac caaagcccgc
     2761 agagcggcat aaggacgaca gcaggggtct tgtgcttcct gggtacaagt acctcggacc
     2821 cttcaacgga ctcgacaagg gagagccggt caacgaggca gacgccgcgg ccctcgagca
     2881 cgacaaagcc tacgaccggc agctcgacag cggagacaac ccgtacctca agtacaacca
     2941 cgccgacgcg gagtttcagg agcgccttaa agaagatacg tcttttgggg gcaacctcgg
     3001 acgagcagtc ttccaggcga aaaagagggt tcttgaacct ctgggcctgg ttgaggaacc
     3061 tgttaaggcg gctccgggaa aaaagaggcc ggtagagcac tctcctgtgg agccagactc
     3121 ctcctcggga accggaaagg cgggccagca gcctgcaaga aaaagattga attttggtca
     3181 gactggagac gcagactcag tacctgaccc ccagcctctc atgcaccacc accaccacca
     3241 cggacagcca ccagcagccc cctctggtct gggaactaat acgctggcta caggcagtgg
     3301 cgcaccactg gcagacaata acgagggcgc cgacggagtg ggtaattcct cgggaaattg
     3361 gcattgcgat tccacatggc tgggcgacag agtcatcacc accagcaccc gaacctgggc
     3421 cctgcccacc tacaacaacc acctctacaa acaaatttcc agccaatcag gagcctcgaa
     3481 cgacaatcac tactttggct acagcacccc ttgggggtat tttgacttca acagattcca
     3541 ctgccacttt tcaccacgtg actggcaaag actcatcaac aacaactggg gattccgacc
     3601 caagagactc aacttcaagc tctttaacat tcaagtcaaa gaggtcacgc agaatgacgg
     3661 tacgacgacg attgccaata accttaccag cacggttcag gtgtttactg actcggagta
     3721 ccagctcccg tacgtcctcg gctcggcgca tcaaggatgc ctcccgccgt tcccagcaga
     3781 cgtcttcatg gtgccacagt atggatacct caccctgaac aacgggagtc aggcagtagg
     3841 acgctcttca ttttactgcc tggagtactt tccttctcag atgctgcgta ccggaaacaa
     3901 ctttaccttc agctacactt ttgaggacgt tcctttccac agcagctacg ctcacagcca
     3961 gagtctggac cgtctcatga atcctctcat cgaccagtac ctgtattact tgagcagaac
     4021 aaacactcca agtggaacca ccacgcagtc aaggcttcag ttttctcagg ccggagcgag
     4081 tgacattcgg gaccagtcta ggaactggct tcctggaccc tgttaccgcc agcagcgagt
     4141 atcaaagaca tctgcggata acaacaacag tgaatactcg tggactggag ctaccaagta
     4201 ccacctcaat ggcagagact ctctggtgaa tccgggcccg gccatggcaa gccacaagga
     4261 cgatgaagaa aagttttttc ctcagagcgg ggttctcatc tttgggaagc aaggctcaga
     4321 gaaaacaaat gtggacattg aaaaggtcat gattacagac gaagaggaaa tcaggacaac
     4381 caatcccgtg gctacggagc agtatggttc tgtatctacc aacctccaga gaggcaacag
     4441 acaagcagct accgcagatg tcaacacaca aggcgttctt ccaggcatgg tctggcagga
     4501 cagagatgtg taccttcagg ggcccatctg ggcaaagatt ccacacacgg acggacattt
     4561 tcacccctct cccctcatgg gtggattcgg acttaaacac cctcctccac agattctcat
     4621 caagaacacc ccggtacctg cgaatccttc gaccaccttc agtgcggcaa agtttgcttc
     4681 cttcatcaca cagtactcca cgggacaggt cagcgtggag atcgagtggg agctgcagaa
     4741 ggaaaacagc aaacgctgga atcccgaaat tcagtacact tccaactaca acaagtctgt
     4801 taatgtggac tttactgtgg acactaatgg cgtgtattca gagcctcgcc ccattggcac
     4861 cagatacctg actcgtaatc tgtaattgct tgttaatcaa taaaccgttt aattcgtttc
     4921 agttgaactt tggtctctgc gtatttcttt cttatctagt ttccatgctc tagaactagt
     4981 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     5041 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     5101 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     5161 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     5221 gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg
     5281 ttaaattttt gttaaatcag ctcatttttt taaccaatag gccgaaatcg gcaaaatccc
     5341 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5401 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5461 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5521 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5581 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     5641 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     5701 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5761 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5821 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5881 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5941 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6001 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6061 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6121 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6181 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6241 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6301 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6361 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6421 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6481 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6541 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6601 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6661 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6721 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6781 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6841 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6901 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6961 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7021 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7081 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7141 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7201 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7261 agaaagcgcc acgct
//
