LOCUS       sequence-244967-        6324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW
  TITLE     Optimized large-scale production of high titer lentivirus vector 
            pseudotypes.
  JOURNAL   J Virol Methods. 2004 Dec 15. 122(2):131-9.
  PUBMED    15542136
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol 
            Methods. 2004 Dec 15. 122(2):131-9."
COMMENT     Sequence Label: pHCMV-LCMV-WE
FEATURES             Location/Qualifiers
     source          1..6324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          316..888
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2553..2572)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2618..2673
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2672..2691)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3031..3047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3031..3047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3044..3066)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3055..3071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3079..3085,3086..3103,3104..3109))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3124..3145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3262..3279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3433..4021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3513..3532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4192..4983,4984..5052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4815..4834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5053..5157)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5183..5638)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5320..5341)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5532..5551
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5765..5787
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5779..5796
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5780..5796
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5806..5825
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5806..5824
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5945..6324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gtcgaccgat
      301 cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta
      361 aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt
      421 cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga
      481 caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc
      541 ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt
      601 ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt
      661 tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc
      721 tggcgtggaa atattcttat tggtagaaac aactacaccc tggtcatcat cctgcctttc
      781 tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa
      841 ccgggcccct ctgctaacca tgttcatgcc ttcttctctt tcctacagct cctgggcaac
      901 gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tcctcgacgg atccgccatg
      961 ggtcagattg tgacaatgtt tgaggctttg cctcacatca ttgatgaggt catcaacatt
     1021 gtcattattg tgctcattat aatcacgagc atcaaagctg tgtacaattt cgccacctgt
     1081 gggatattag cactggtcag cttccttttt ctggctggta ggtcctgtgg catgtacggc
     1141 cttaatggtc ccgatatcta taaaggggtt taccagttca aatcagtgga gtttgatatg
     1201 tctcacttaa atctgacgat gcccaatgcg tgctcagtca acaactctca tcactacatc
     1261 agtatgggaa gctctggact ggagctaact ttcaccaacg attccatcct taatcacaac
     1321 ttctgcaact taacctccgc tttcaacaaa aagtcttttg accatacact catgagtata
     1381 gtctcgagtc tacacctcag tatcagaggg aattccaact acaaagcagt gtcttgtgat
     1441 tttaacaatg gcatcaccat tcaatacaac ttgtcatttt cggacccaca gagcgctata
     1501 agccagtgta ggactttcag aggtagagtc ttggacatgt ttagaactgc ctttggagga
     1561 aagtacatga gaagtggctg gggctggaca ggttcagatg gcaagaccac ttggtgcagc
     1621 caaacaagct atcagtacct aatcatacaa aacaggactt gggaaaacca ctgtagatat
     1681 gcaggccctt ttgggatgtc tagaatcctc tttgctcagg aaaagacaaa gtttctcact
     1741 aggagacttg caggcacatt cacctggacc ctgtcagact cctcaggagt agaaaatcca
     1801 ggtggttatt gcctgaccaa atggatgatc cttgctgcag agctcaaatg ttttgggaat
     1861 acagctgttg caaaatgtaa tgtcaatcat gatgaagagt tctgtgacat gctacgacta
     1921 attgattaca acaaggctgc cctgagtaag ttcaagcaag atgtagagtc tgccttgcat
     1981 gtattcaaaa caacagtaaa ttctctgatt tccgatcagc tgttgatgag gaatcatcta
     2041 agagatctaa tgggggtacc atactgtaat tactcaaagt tctggtatct ggaacatgct
     2101 aagactggtg agactagtgt acccaagtgt tggcttgtca ctaatggctc ctacttgaat
     2161 gagacccatt ttagtgatca aatcgaacaa gaagcagata acatgatcac agagatgttg
     2221 aggaaggact acataaaaag acaagggagt actcctttag ccttaatgga tcttttgatg
     2281 ttttcaacat cagcatatct gatcagcatc tttctgcatc ttgtgaagat accaacacat
     2341 agacacataa agggcggttc atgtccaaag ccacatcgct tgaccaacaa ggggatctgt
     2401 agttgtggtg cattcaaggt gcctggtgta aaaactatct ggaaaagacg ctgataagga
     2461 tccgtcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     2521 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     2581 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     2641 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     2701 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     2761 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     2821 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     2881 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     2941 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     3001 ctcgacggat cggccgcaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     3061 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3121 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3181 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3241 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3841 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3901 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3961 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4021 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4081 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4141 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4201 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4261 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4321 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4381 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4441 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4501 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4561 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4621 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4681 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4741 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4801 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4861 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4921 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4981 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5041 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5101 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5161 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     5221 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5281 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5341 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5401 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5461 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5521 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5581 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     5641 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     5701 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     5761 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     5821 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gagagcttgg cccattgcat
     5881 acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac attaccgcca
     5941 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     6001 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     6061 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     6121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     6181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     6241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     6301 gtattagtca tcgctattac catg
//
