LOCUS       sequence-244969-       11083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, 
            mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   O'Geen H, Bates SL, Carter SS, Nisson KA, Halmai J, Fink KD, Rhie 
            SK, Farnham PJ, Segal DJ
  TITLE     Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic memory in
            a context-dependent manner.
  JOURNAL   Epigenetics Chromatin. 2019 May 3;12(1):26. doi: 
            10.1186/s13072-019-0275-8.
  PUBMED    31053162
REFERENCE   2  (bases 1 to 11083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Epigenetics
            Chromatin."; date: "2019-05-3"; volume: "12(1):26. doi"; pages: " 
            10.1186/s13072-019-0275-8"
COMMENT     Sequence Label: DNMT3L-dCas9
FEATURES             Location/Qualifiers
     source          1..11083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     654..678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             775..795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             814..879
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      908..917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2128..2154,2155..2157,2158..4641,4642..4647,4648..4713,
                     4714..4716,4717..6231)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2128..2154,2155..2157,2158..4644,4645..4647,
                     4648..6231)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2128..6231
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6307..6354
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6373..6429
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6493..6512)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6537..6585
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6787..7215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6874..6893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7084..7105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7224..7244)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7229..7558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7409..7544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7471..7490
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7625..8419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7679..7698)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8289..8308
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8595..8716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8632..8651)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8686..8705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8765..8781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8765..8781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8778..8800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8789..8805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8813..8819,8820..8837,8838..8843))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8858..8879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8996..9013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9167..9755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9247..9266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9926..10717,10718..10786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10549..10568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10787..10891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10966..10985)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      661 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      721 caccgggacc gatccagcct ccggactcta gaggatcgaa cccttgccac catgcccaag
      781 aagaagagga aggtgggagg ctccggagga agcgactata aggaccacga cggagactac
      841 aaggatcatg atattgatta caaagacgat gacgataagg gtggcgggtc cggcggtgga
      901 tccggtacca tggcggccat cccagccctg gacccagagg ccgagcccag catggacgtg
      961 attttggtgg gatccagtga gctctcaagc tccgtttcac ccgggacagg cagagatctt
     1021 attgcatatg aagtcaaggc taaccagcga aatatagaag acatctgcat ctgctgcgga
     1081 agtctccagg ttcacacaca gcaccctctg tttgagggag ggatctgcgc cccatgtaag
     1141 gacaagttcc tggatgccct cttcctgtac gacgatgacg ggtaccaatc ctactgctcc
     1201 atctgctgct ccggagagac gctgctcatc tgcggaaacc ctgattgcac ccgatgctac
     1261 tgcttcgagt gtgtggatag cctggtcggc cccgggacct cggggaaggt gcacgccatg
     1321 agcaactggg tgtgctacct gtgcctgccg tcctcccgaa gcgggctgct gcagcgtcgg
     1381 aggaagtggc gcagccagct caaggccttc tacgaccgag agtcggagaa tccccttgag
     1441 atgttcgaaa ccgtgcctgt gtggaggaga cagccagtcc gggtgctgtc cctttttgaa
     1501 gacatcaaga aagagctgac gagtttgggc tttttggaaa gtggttctga cccgggacaa
     1561 ctgaagcatg tggttgatgt cacagacaca gtgaggaagg atgtggagga gtggggaccc
     1621 ttcgatcttg tgtacggcgc cacacctccc ctgggccaca cctgtgaccg tcctcccagc
     1681 tggtacctgt tccagttcca ccggctcctg cagtacgcac ggcccaagcc aggcagcccc
     1741 aggcccttct tctggatgtt cgtggacaat ctggtgctga acaaggaaga cctggacgtc
     1801 gcatctcgct tcctggagat ggagccagtc accatcccag atgtccacgg cggatccttg
     1861 cagaatgctg tccgcgtgtg gagcaacatc ccagccataa ggagcaggca ctgggctctg
     1921 gtttcggaag aagaattgtc cctgctggcc cagaacaagc agagctcgaa gctcgcggcc
     1981 aagtggccca ccaagctggt gaagaactgc tttctccccc taagagaata tttcaagtat
     2041 ttttcaacag aactcacttc ctctttaagt accggaggga gtggtggaag cggcggttct
     2101 ggtggctcag gcggcagcgg ccggccaatg gacaagaagt actccattgg gctcgctatc
     2161 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa
     2221 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc
     2281 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga
     2341 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct
     2401 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa
     2461 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag
     2521 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg
     2581 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag
     2641 ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact
     2701 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc
     2761 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg
     2821 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac
     2881 tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac
     2941 gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg
     3001 gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag
     3061 atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac
     3121 ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc
     3181 ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa
     3241 ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta
     3301 aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc
     3361 caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc
     3421 tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat
     3481 gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag
     3541 accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc
     3601 atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac
     3661 tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca
     3721 gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc
     3781 ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag
     3841 attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg
     3901 ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag
     3961 aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg
     4021 attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc
     4081 aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga
     4141 gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaacagg
     4201 aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca
     4261 caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca
     4321 gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg
     4381 ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag
     4441 aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg
     4501 gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc
     4561 tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat
     4621 cggctctccg actacgacgt ggatgctatc gtgccccagt cttttctcaa agatgattct
     4681 attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga taacgtcccc
     4741 tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg
     4801 atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg
     4861 gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg
     4921 gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga
     4981 gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag
     5041 ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca
     5101 gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga
     5161 gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag
     5221 gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca
     5281 ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa
     5341 atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag
     5401 gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc
     5461 ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac
     5521 ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa
     5581 gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga
     5641 tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa
     5701 aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa
     5761 cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa
     5821 tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat
     5881 aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag
     5941 caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt
     6001 tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac
     6061 ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata
     6121 gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca
     6181 attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cggtggtagt
     6241 ggaggttcag gaggatccgg ggggagcgga gggagcgcta gcggtggagg ctccggaggt
     6301 ggatctaaaa ggccggcggc cacgaaaaag gccggtcagg caaaaaagaa aaagggtggt
     6361 agtggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     6421 cctggacctg cggccgcatg aaagggttcg atccctaccg gttagtaatg agtttaaacg
     6481 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     6541 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc
     6601 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     6661 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     6721 ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg cagctggggc tctagggggt
     6781 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6841 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6901 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6961 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7021 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7081 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7141 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7201 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7381 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7561 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     7621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     7681 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     7741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     7801 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     8041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     8101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     8161 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8401 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc
     8461 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     8521 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     8581 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8641 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8701 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     8761 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8821 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8881 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8941 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9001 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9061 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9361 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9601 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10921 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
    10981 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
    11041 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gac
//
