LOCUS       sequence-244971-       11080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, 
            mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   O'Geen H, Bates SL, Carter SS, Nisson KA, Halmai J, Fink KD, Rhie 
            SK, Farnham PJ, Segal DJ
  TITLE     Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic memory in
            a context-dependent manner.
  JOURNAL   Epigenetics Chromatin. 2019 May 3;12(1):26. doi: 
            10.1186/s13072-019-0275-8.
  PUBMED    31053162
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Epigenetics
            Chromatin."; date: "2019-05-3"; volume: "12(1):26. doi"; pages: " 
            10.1186/s13072-019-0275-8"
COMMENT     Sequence Label: dCas9-DNMT3L
FEATURES             Location/Qualifiers
     source          1..11080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     654..678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             775..795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             814..879
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(964..990,991..993,994..3477,3478..3483,3484..3549,
                     3550..3552,3553..5067)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(964..990,991..993,994..3480,3481..3483,3484..5067)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             964..5067
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6304..6351
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6370..6426
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6490..6509)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6534..6582
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6784..7212
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6871..6890)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7081..7102
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7221..7241)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7226..7555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7406..7541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7468..7487
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7622..8416
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7676..7695)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8286..8305
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8592..8713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8629..8648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8683..8702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8762..8778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8762..8778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8775..8797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8786..8802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8810..8816,8817..8834,8835..8840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8855..8876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8993..9010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9164..9752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9244..9263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9923..10714,10715..10783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10546..10565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10784..10888)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10963..10982)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      661 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      721 caccgggacc gatccagcct ccggactcta gaggatcgaa cccttgccac catgcccaag
      781 aagaagagga aggtgggagg ctccggagga agcgactata aggaccacga cggagactac
      841 aaggatcatg atattgatta caaagacgat gacgataagg gtggcgggtc cggcggtgga
      901 tccggtaccg gagggagtgg tggaagcggc ggttctggtg gctcaggcgg cagcggccgg
      961 ccaatggaca agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc
     1021 gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat
     1081 cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc
     1141 gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc
     1201 tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat
     1261 aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt
     1321 ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg
     1381 aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg
     1441 catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc
     1501 gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac
     1561 ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc
     1621 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt
     1681 aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc
     1741 gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg
     1801 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc
     1861 attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct
     1921 agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc
     1981 agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac
     2041 gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc
     2101 ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg
     2161 cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg
     2221 cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag
     2281 attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat
     2341 tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag
     2401 gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat
     2461 aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca
     2521 gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc
     2581 ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt
     2641 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa
     2701 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa
     2761 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt
     2821 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac
     2881 gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg
     2941 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc
     3001 ctggattttc ttaagtccga tggatttgcc aacaggaact tcatgcagtt gatccatgat
     3061 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
     3121 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
     3181 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
     3241 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa
     3301 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
     3361 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
     3421 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat
     3481 gctatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
     3541 tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
     3601 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
     3661 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg
     3721 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
     3781 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
     3841 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
     3901 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
     3961 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
     4021 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
     4081 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
     4141 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
     4201 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
     4261 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
     4321 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
     4381 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc
     4441 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
     4501 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
     4561 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag
     4621 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
     4681 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
     4741 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
     4801 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
     4861 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc
     4921 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca
     4981 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
     5041 atcgacctct ctcagctcgg tggagacggt ggtagtggag gttcaggagg atccgggggg
     5101 agcggaggga gcgctagcat ggcggccatc ccagccctgg acccagaggc cgagcccagc
     5161 atggacgtga ttttggtggg atccagtgag ctctcaagct ccgtttcacc cgggacaggc
     5221 agagatctta ttgcatatga agtcaaggct aaccagcgaa atatagaaga catctgcatc
     5281 tgctgcggaa gtctccaggt tcacacacag caccctctgt ttgagggagg gatctgcgcc
     5341 ccatgtaagg acaagttcct ggatgccctc ttcctgtacg acgatgacgg gtaccaatcc
     5401 tactgctcca tctgctgctc cggagagacg ctgctcatct gcggaaaccc tgattgcacc
     5461 cgatgctact gcttcgagtg tgtggatagc ctggtcggcc ccgggacctc ggggaaggtg
     5521 cacgccatga gcaactgggt gtgctacctg tgcctgccgt cctcccgaag cgggctgctg
     5581 cagcgtcgga ggaagtggcg cagccagctc aaggccttct acgaccgaga gtcggagaat
     5641 ccccttgaga tgttcgaaac cgtgcctgtg tggaggagac agccagtccg ggtgctgtcc
     5701 ctttttgaag acatcaagaa agagctgacg agtttgggct ttttggaaag tggttctgac
     5761 ccgggacaac tgaagcatgt ggttgatgtc acagacacag tgaggaagga tgtggaggag
     5821 tggggaccct tcgatcttgt gtacggcgcc acacctcccc tgggccacac ctgtgaccgt
     5881 cctcccagct ggtacctgtt ccagttccac cggctcctgc agtacgcacg gcccaagcca
     5941 ggcagcccca ggcccttctt ctggatgttc gtggacaatc tggtgctgaa caaggaagac
     6001 ctggacgtcg catctcgctt cctggagatg gagccagtca ccatcccaga tgtccacggc
     6061 ggatccttgc agaatgctgt ccgcgtgtgg agcaacatcc cagccataag gagcaggcac
     6121 tgggctctgg tttcggaaga agaattgtcc ctgctggccc agaacaagca gagctcgaag
     6181 ctcgcggcca agtggcccac caagctggtg aagaactgct ttctccccct aagagaatat
     6241 ttcaagtatt tttcaacaga actcacttcc tctttatccg gtggaggctc cggaggtgga
     6301 tctaaaaggc cggcggccac gaaaaaggcc ggtcaggcaa aaaagaaaaa gggtggtagt
     6361 ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
     6421 ggacctgcgg ccgcatgaaa gggttcgatc cctaccggtt agtaatgagt ttaaacgggg
     6481 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     6541 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     6601 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     6661 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     6721 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     6781 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6841 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6901 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6961 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7021 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7081 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7141 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7201 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7381 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7441 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7501 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7561 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     7621 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     7681 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     7741 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     7801 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     7861 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     7921 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     7981 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     8041 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     8101 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     8161 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     8221 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     8281 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     8341 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     8401 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     8461 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8521 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8581 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8701 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10921 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    10981 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    11041 gagcaaaatt taagctacaa caaggcaagg cttgaccgac
//
