LOCUS       sequence-244973-        8885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses mammalian FLAG-NUFIP1 W40A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Wyant GA, Abu-Remaileh M, Frenkel EM, Laqtom NN, Dharamdasani V, 
            Lewis CA, Chan SH, Heinze I, Ori A, Sabatini DM
  TITLE     NUFIP1 is a ribosome receptor for starvation-induced ribophagy.
  JOURNAL   Science. 2018 May 18;360(6390):751-758. doi: 
            10.1126/science.aar2663. Epub 2018 Apr 26.
  PUBMED    29700228
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aar2663"; journalName: "Science"; date: 
            "2018-05-18- 18"; volume: "360"; issue: "6390"; pages: "751-758"
COMMENT     Sequence Label: pLJM60 FLAG NUFIP1 W40A
FEATURES             Location/Qualifiers
     source          1..8885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     222..242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        224..242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        270..496
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             497..677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    724..849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1346..1579
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1764..1808
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2115..2318
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2269..2289
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2315..2339
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2549..2567
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2549..2566
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2580..2599
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4248..4365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4414..4924
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4843..4861
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4946..5545
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4946..4965)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5442..5462
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5673..5906
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5978..6099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6015..6034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6069..6088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6139..6274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6294..6313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6295..6313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6323..6339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6332..6354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6481..6936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6568..6587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6778..6799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6962..7066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7067..7135,7136..7927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7285..7304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8098..8686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8587..8606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8840..8857
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
      241 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
      301 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
      361 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
      421 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
      481 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      661 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      721 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      781 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
      841 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
      901 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
      961 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1021 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1081 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1141 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     1201 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1261 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1321 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1381 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1441 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1501 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1561 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1621 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1681 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1741 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1801 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     1861 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     1921 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     1981 cccatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2041 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2101 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2161 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2221 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2281 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2341 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     2401 tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta
     2461 ccgcctatag agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat
     2521 acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt
     2581 tgcctttctc tccacaggtg tccactccca ggtccaactg cacctcggtt ctatcgattg
     2641 aattcaccat ggaaatggac tacaaggatg acgatgacaa gggtggtggt ggtggtgcgt
     2701 cgacgatggc tgagccgact agtgatttcg agactcctat cgggtggcat gcgtctcccg
     2761 agctgactcc cacgttaggg cccctgagcg acactgcccc gccgcgggac agctggatgt
     2821 tcgcggcaat gctgccgcca ccgccaccac cacttacgtc ctcgcttccc gcagccgggt
     2881 caaagccttc ctctgagtcg cagcccccca tggaggccca gtctctcccc ggggctccgc
     2941 cccccttcga cgcccagatt cttcccgggg cgcaaccccc cttcgacgcc cagtctcccc
     3001 ttgattctca gcctcaaccc agcggccagc cttggaattt ccatgcttcc acatcgtggt
     3061 attggagaca gtcttctgat aggtttcctc ggcatcagaa gtccttcaac cctgcagtta
     3121 aaaattctta ttatccacga aagtatgatg caaaattcac agacttcagc ttacctccca
     3181 gtagaaaaca gaaaaaaaag aaaagaaagg aaccagtttt tcactttttt tgtgatacct
     3241 gtgatcgtgg ttttaaaaat caagaaaagt atgacaaaca catgtctgaa catacaaaat
     3301 gccctgaatt agattgctct tttactgcac acgagaagat tgtccagttc cattggagaa
     3361 atatgcatgc tcctggcatg aagaagatca agttagacac tccagaggaa attgcacggt
     3421 ggagggaaga aagaaggaaa aactatccaa ctctggccaa tattgaaagg aagaagaagt
     3481 taaaacttga aaaggagaag agaggagcag tattgacaac aacacaatat ggcaagatga
     3541 aggggatgtc cagacattca caaatggcaa agatcagaag tcctggcaag aatcacaaat
     3601 ggaaaaacga caattctaga cagagagcag tcactggatc aggcagtcac ttgtgtgatt
     3661 tgaagctaga aggtccaccg gaggcaaatg cagatcctct tggtgttttg ataaacagtg
     3721 attctgagtc tgataaggag gagaaaccac aacattctgt gatacccaag gaagtgacac
     3781 cagccctatg ctcactaatg agtagctatg gcagtctttc agggtcagag agtgagccag
     3841 aagaaactcc catcaagact gaagcagacg ttttggcaga aaaccaggtt cttgatagca
     3901 gtgctcctaa gagtccaagt caagatgtta aagcaactgt tagaaatttt tcagaagcca
     3961 agagtgagaa ccgaaagaaa agctttgaaa aaacaaaccc taagaggaaa aaagattatc
     4021 acaactatca aacgttattc gaaccaagaa cacaccatcc atatctcttg gaaatgcttc
     4081 tagctccgga cattcgacat gaaagaaatg tgattttgca gtgtgttcgg tacatcatca
     4141 aaaaagactt ttttggactg gatactaatt ctgcgaaaag taaagatgta taggcggccg
     4201 cagtgagaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
     4261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     4381 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
     4441 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     4501 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     4561 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     4621 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     4681 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     4741 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     4801 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     4861 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     4921 ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     4981 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5041 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5101 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5161 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5221 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5281 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5341 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5401 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5461 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5521 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     5581 gagcgcacga ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5641 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5701 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5881 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5941 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6121 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6241 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6301 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6901 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7021 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7081 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7141 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7201 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7261 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7321 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7381 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7441 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7501 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7561 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7621 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7681 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7741 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7801 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7861 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7921 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8701 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8761 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8821 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8881 caaac
//
