LOCUS       sequence-24498-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J
  TITLE     TGFbeta primes breast tumors for lung metastasis seeding through 
            angiopoietin-like 4.
  JOURNAL   Cell. 2008 Apr 4. 133(1):66-77.
  PUBMED    18394990
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Apr 4. 133(1):66-77."
COMMENT     Sequence Label: pRetroSuper shSmad4J hygro
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(111..127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(142..558)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(158..174)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(190..212)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tttggatttg ggatcttata agttctgtat gagaccacag atccccggtg tgcagttgga
       61 atgtattcaa gagatacatt ccaactgcac acctttttgg aaaagcttat cgataccgtc
      121 gacctcgaga gatctaattc cggcgcctag agaaggagtg agggctggat aaagggagga
      181 tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg gaggaagagg
      241 aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa aagccaaggc
      301 ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac
      361 cttgatctta acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac
      421 gttaaaggtt ggccattctg cagagcagaa ggtaacccaa cgtctcttct tgacatctac
      481 cgactggttg tgagcgatcc gctcgacatc tttccagtga cctaaggtca aacttaaggg
      541 agtggtaaca gtctggccct aattttcaga caaatacaga aacacagtca gacagagaca
      601 acacagaacg atgctgcagc gctgcagcag acaagacgcg cggcttcggt tccaaaccga
      661 aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta ccagaaccac
      721 atatcctgac ggggtcggat tccacatcga ctcccttcct caggtcgggc cacaaaaacg
      781 gcccccaaag tccctgggac gtctcccagg gttgcggccg ggtgttcaga actcgtcagt
      841 tccaccacgg ggtccgccag atacagagct agttagctaa ctagtacaga cgcaggcgca
      901 aacattagat gccggcacaa acacccaaac gaacgncna
//
