LOCUS       sequence-244983-        1808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes permuteposon P2 for MuA transposase-mediated circular 
            permutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Atkinson JT, Wu B, Segatori L, Silberg JJ
  TITLE     Overcoming component limitations in synthetic biology through 
            transposon-mediated protein engineering.
  JOURNAL   Methods Enzymol. 2019;621:191-212. doi: 10.1016/bs.mie.2019.02.014. 
            Epub 2019 Apr 2.
  PUBMED    31128779
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2019"; volume: "621:191-212. doi"; pages: " 
            10.1016/bs.mie.2019.02.014. Epub 2019 Apr 2"
COMMENT     Sequence Label: pMT-P2
FEATURES             Location/Qualifiers
     source          1..1808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..820
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALTVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(78..97)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(1097..1685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1177..1196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(join(1802..1808,1..13))
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tgttatgagc catattcaac gggaaacgtc ttgctctagg ccgcgattaa attccaacat
       61 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      121 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      181 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
      241 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
      301 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
      361 tattgttgat gcgctgacag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
      421 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
      481 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
      541 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
      601 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
      661 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
      721 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
      781 taaattgcag tttcatttga tgctcgatga gtttttctaa ggtacctttt cgcatttatc
      841 gtgaaacgct ttcgcatttt gagtgaggta tatgagatct gaagcggcgc acgaaaaacg
      901 cgaaagcgtt tcacgataaa tgcgaaaaaa aggatctcaa gaagatcctt tgattttcgg
      961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1021 gataacgcag gaaagaacat gtgagcaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1501 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc catggctcag tggaacgaaa
     1741 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1801 acaagggg
//
