LOCUS       sequence-245052-       10288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an 
            intein-chitin-binding domain fusion for purification of untagged 
            full-length protein, with or without a fluorescent label on the 
            C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Liu X, Schuessler PJ, Sahoo A, Walker SE
  TITLE     Reconstitution and analyses of RNA interactions with eukaryotic 
            translation initiation factors and ribosomal preinitiation 
            complexes.
  JOURNAL   Methods. 2019 Mar 26. pii: S1046-2023(18)30395-5. doi: 
            10.1016/j.ymeth.2019.03.024.
  PUBMED    30926531
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2019 Mar 26. pii: S1046-2023(18)30395-5. doi: 
            10.1016/j.ymeth.2019.03.024."
COMMENT     Sequence Label: pSW149
FEATURES             Location/Qualifiers
     source          1..10288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2951..3769
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             4225..4236
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4346..4501
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(4566..4584)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4580..4627
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4687..4791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4792..4860,4861..5652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5010..5029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5694..6207)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5889..5910)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6101..6120
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6318..6906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6807..6826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7060..7077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7099..7118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7259..7281
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7275..7466)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8023..9105)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9067..9086
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9106..9183)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      9336..9422
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9519..9605
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9702..9788
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9885..9971
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10068..10154
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gacagacgaa actgctcacc cgacacaatc
      121 tgcttcaaag caggaatctg ctgctctgaa acagacaggc gatgaccagc aggaatctca
      181 gcaacaacgt ggctacacca actacaacaa tggtagcaac tatacccaaa agaagcctta
      241 caacagcaac agacctcatc agcaaagagg tggtaaattt ggaccaaaca gatataacaa
      301 ccgtggcaac tataatggtg gcggtagttt cagaggtgga cacatgggag ccaacagctc
      361 aaacgtgcca tggactggtt actataataa ctaccccgtt tactaccagc cccagcaaat
      421 ggcggccgct ggtagtgccc ctgctaatcc aattcctgtc gaagagaagt cacctgttcc
      481 aactaagata gaaatcacta ccaagtctgg tgaacactta gatttgaaag aacagcataa
      541 agccaagcta cagtctcagg aaagatctac tgtgtctccg caaccagagt caaagttaaa
      601 agaaacttct gattctactt ctacttctac tccaactcct accccttcca ctaatgactc
      661 taaggccagt tctgaagaaa atatatctga agctgaaaag acaagaagaa atttcatcga
      721 gcaagttaaa cttcgtaaag ctgctttaga aaagaagaga aaggagcaac ttgaaggttc
      781 tagtggcaac aataatattc caatgaagac taccccagaa aatgtggaag agaagggatc
      841 ggacaaacct gaggtaaccg aaaaaaccaa gcctgctgaa gaaaaatctg ctgagccaga
      901 agttaagcaa gaaactccag ctgaagaagg tgaacaagga gaaaagggcc aaataaagga
      961 agaatccact ccaaaggtgt taacctttgc tgaacgtttg aagttgaaga aacaacaaaa
     1021 agaacgagaa gaaaaaacgg aggggaagga aaacaaggaa gtgccagtac aggaagagac
     1081 taagagcgcc atcgagtctg ctcctgtgcc tcccagtgaa caggttaaag aagaaactga
     1141 ggtcgccgaa accgaacagt caaacattga tgaatctgca acaactccgg ctatccctac
     1201 taaatctgat gaagctgaag ctgaagttga ggctgaagca ggtgatgctg gaacaaagat
     1261 tggacttgaa gctgaaatcg aaactacaac tgatgaaact gatgatggta caaacaccgt
     1321 ttctcacata ttgaatgtat tgaaagatgc tacaccaatt gaagatgtct tttcttttaa
     1381 ctatccagaa ggtattgaag gtcctgatat caaatacaag aaagaacacg tcaaatatac
     1441 atatggccca actttcttgc ttcaatttaa ggacaaacta aacgtcaaag cggacgccga
     1501 atgggtccaa agcactgctt ctaaaattgt cattccacca ggaatgggca gaggaaatag
     1561 atctagagat tctggcagat tcggcaataa ttctagtcgt ggccatgact ttagaaatac
     1621 ctcagtgaga aatatggatg acagagctaa ttcaagaact tcatcaaaga gaagatcaaa
     1681 gagaatgaat gacgacagga gatctaatag atcttataca tcaagaagag accgcgagag
     1741 aggctcctac agaaatgaag agaaaagaga ggacgataaa ccaaaggaag aagttgctcc
     1801 acttgttcca agtgctaata ggtgggtgcc aaaattcaag tctaaaaaga ctgaaaagaa
     1861 gctagcccct gacggaaaga ccgaactatt ggacaaggat gaagttgaaa gaaagatgaa
     1921 gtcactactg aataaactta ctttggaaat gttcgatgct atttcatctg aaattttggc
     1981 tattgcaaac atatcagtat gggaaacaaa tggtgaaacg ttaaaggctg tgatagaaca
     2041 gattttctta aaggcctgtg acgaacccca ttggtcttct atgtacgcgc aattatgtgg
     2101 taaagtagtt aaagaattaa acccagatat aacagatgaa actaatgaag gtaagacagg
     2161 tccaaaattg gttttgcatt acttggttgc tagatgtcat gccgaatttg acaagggttg
     2221 gaccgataaa ttacccacaa acgaagatgg tactccatta gaacctgaga tgatgtcaga
     2281 agagtactat gcagctgctt ctgctaagag aagaggttta ggtttggttc gtttcatcgg
     2341 tttcctatac cgtctgaatc tgttgactgg aaagatgatg ttcgaatgtt tccgtagact
     2401 aatgaaggat ttgacggatt ctccatctga agaaactcta gagtccgttg ttgaattgtt
     2461 aaatactgtt ggtgaacaat ttgagaccga tagtttcaga acgggtcaag caacattgga
     2521 gggttcgcaa ttgctcgata gtttgttcgg tattttagac aatattattc aaactgctaa
     2581 gatttctagt agaatcaagt tcaagttgat cgacattaaa gaattaaggc acgacaagaa
     2641 ctggaacagt gataagaagg acaacggtcc taagaccatt caacagattc atgaggaaga
     2701 ggagagacaa cgtcaattga agaataattc aagatctaat tcaagacgta cgaacaactc
     2761 cagtaataga cactctttca gaagggacgc tcctcctgca tcaaaagata gctttatcac
     2821 aaccagaaca tattctcaaa gaaatagcca aagggctcct cctccaaagg aagaaccagc
     2881 tgcaccaact tctaccgcaa caaatatgtt cagtgcatta atgggagaaa gtgatgacga
     2941 agagcccggg tgctttgcca agggtaccaa tgttttaatg gcggatgggt ctattgaatg
     3001 tattgaaaac attgaggttg gtaataaggt catgggtaaa gatggcagac ctcgtgaggt
     3061 aattaaattg cccagaggaa gagaaactat gtacagcgtc gtgcagaaaa gtcagcacag
     3121 agcccacaaa agtgactcaa gtcgtgaagt gccagaatta ctcaagttta cgtgtaatgc
     3181 gacccatgag ttggttgtta gaacacctcg tagtgtccgc cgtttgtctc gtaccattaa
     3241 gggtgtcgaa tattttgaag ttattacttt tgagatgggc caaaagaaag cccccgacgg
     3301 tagaattgtt gagcttgtca aggaagtttc aaagagctac ccaatatctg aggggcctga
     3361 gagagccaac gaattagtag aatcctatag aaaggcttca aataaagctt attttgagtg
     3421 gactattgag gccagagatc tttctctgtt gggttcccat gttcgtaaag ctacctacca
     3481 gacttacgct ccaattcttt atgagaatga ccactttttc gactacatgc aaaaaagtaa
     3541 gtttcatctc accattgaag gtccaaaagt acttgcttat ttacttggtt tatggattgg
     3601 tgatggattg tctgacaggg caactttttc ggttgattcc agagatactt ctttgatgga
     3661 acgtgttact gaatatgctg aaaagttgaa tttgtgcgcc gagtataagg acagaaaaga
     3721 accacaagtt gccaaaactg ttaatttgta ctctaaagtt gtcagaggta atggtattcg
     3781 caataatctt aatactgaga atccattatg ggacgctatt gttggcttag gattcttgaa
     3841 ggacggtgtc aaaaatattc cttctttctt gtctacggac aatatcggta ctcgtgaaac
     3901 atttcttgct ggtctaattg attctgatgg ctatgttact gatgagcatg gtattaaagc
     3961 aacaataaag acaattcata cttctgtcag agatggtttg gtttcccttg ctcgttcttt
     4021 aggcttagta gtctcggtta acgcagaacc tgctaaggtt gacatgaatg tcaccaaaca
     4081 taaaattagt tatgctattt atatgtctgg tggagatgtt ttgcttaacg ttctttcgaa
     4141 gtgtgccggc tctaaaaaat tcaggcctgc tcccgccgct gcttttgcac gtgagtgccg
     4201 cggattttat ttcgagttac aagaattgaa ggaagacgat tattatggga ttactttatc
     4261 tgatgattct gatcatcagt ttttgcttgg atcccaggtt gtcgtccatg catgcggtgg
     4321 cctgaccggt ctgaactcag gcctcacgac aaatcctggt gtatccgctt ggcaggtcaa
     4381 cacagcttat actgcgggac aattggtcac atataacggc aagacgtata aatgtttgca
     4441 gccccacacc tccttggcag gatgggaacc atccaacgtt cctgccttgt ggcagcttca
     4501 atgactgcag gaaggggatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     4561 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     4621 ttttttgctg aaaggaggaa ctatatccgg ataactacgt caggtggcac ttttcgggga
     4681 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4741 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4801 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4861 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4921 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4981 ttcccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5041 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5101 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5161 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5221 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5281 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5341 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5401 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5461 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5521 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5581 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5641 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaccccgg
     5701 ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa
     5761 acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     5821 aataggccga aatcggcaaa atcccttata aatcaaaaga atagcccgag atagggttga
     5881 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     5941 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt
     6001 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     6061 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     6121 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     6181 cgcttaatgc gccgctacag ggcgcgtaaa aggatctagg tgaagatcct ttttgataat
     6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7021 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7081 gctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     7141 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     7201 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7261 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     7321 taaagctcat cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc
     7381 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     7441 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
     7501 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
     7561 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
     7621 cagagaaaaa tcactcaggg tcaatgccag ccgaacgcca gcaagacgta gcccagcgcg
     7681 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     7741 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     7801 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     7861 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     7921 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     7981 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     8041 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8101 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     8161 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     8221 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     8281 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
     8341 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     8401 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     8461 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     8521 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     8581 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     8641 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     8701 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     8761 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     8821 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     8881 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     8941 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     9001 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     9061 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     9121 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     9181 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     9241 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgccggcat gccgcccttt
     9301 cgtcttcaag aattaattcc caattcccca ggcatcaaat aaaacgaaag gctcagtcga
     9361 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     9421 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     9481 gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga aaggctcagt
     9541 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     9601 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     9661 gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa cgaaaggctc
     9721 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     9781 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     9841 caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata aaacgaaagg
     9901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     9961 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
    10021 gggcaggacg cccgccataa actgccagga attaattccc caggcatcaa ataaaacgaa
    10081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
    10141 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
    10201 ggcgggcagg acgcccgcca taaactgcca ggaattgggg atcggaatta attcccggtt
    10261 taaaccgggg atctcgatcc cgcgaaat
//
