LOCUS       sequence-245056-        8823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pYPQ256-x3.7
FEATURES             Location/Qualifiers
     source          1..8823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..75
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     309..326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(485..512)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(604..690)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     739..761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     753..770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     754..770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    786..885
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             907..954
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             961..981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1006..5106
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5107..5154
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5371..5436
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6070..6090
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6097..6348
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6349..6402
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     terminator      6598..6850
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6895..7018
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7035..7054)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7036..7054)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7059..7075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7059..7075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7506..8297
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(8390..8823,1..155)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
       61 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      421 cgggcagtga gcgcaacgca attaatacgc gtaccgcgag ccaggaagag tttgtagaaa
      481 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
      541 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
      601 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      661 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
      721 cgcttgcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
      781 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
      841 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctccgaa ttcgcccttc
      901 accatggatt acaaggatga tgatgataag gattacaagg atgatgatga taagatggct
      961 ccaaagaaga agagaaaggt tggaatccac ggagttccag ctgctgataa gaagtactct
     1021 atcggacttg ccatcggaac caactctgtt ggatgggctg ttatcaccga tgagtacaag
     1081 gttccatcta agaagttcaa ggttcttgga aacaccgata gacactctat caagaagaac
     1141 cttatcggtg ctcttctttt cgattctgga gagaccgctg aggctaccag attgaagaga
     1201 accgctagaa gaagatacac cagaagaaag aacagaatct gctaccttca ggaaatcttc
     1261 tctaacgaga tggctaaggt tgatgattct ttcttccaca gacttgagga gtctttcctt
     1321 gttgaggagg ataagaagca cgagagacac ccaatcttcg gaaacatcgt tgatgaggtt
     1381 gcttaccacg agaagtaccc aaccatctac caccttagaa agaagttggt tgattctacc
     1441 gataaggctg atcttagact tatctacctt gctcttgctc acatgatcaa gttcagagga
     1501 cacttcctta tcgagggaga ccttaaccca gataactctg atgttgataa gttgttcatc
     1561 cagcttgttc agacctacaa ccagcttttc gaggagaacc caatcaacgc ttctggagtt
     1621 gatgctaagg ctatcctttc tgctagactt tctaagtctc gtagacttga gaaccttatc
     1681 gctcagcttc caggagagaa gaagaacgga cttttcggaa accttatcgc tctttctctt
     1741 ggacttaccc caaacttcaa gtctaacttc gatcttgctg aggataccaa gttgcagctt
     1801 tctaaggata cctacgatga tgatcttgat aaccttcttg ctcagatcgg agatcagtac
     1861 gctgatcttt tccttgctgc taagaacctt tctgatgcta tccttctttc tgacatcctt
     1921 agagttaaca ccgagatcac caaggctcca ctttctgctt ctatgatcaa gctctacgat
     1981 gagcaccacc aggatcttac ccttttgaag gctcttgtta gacagcagct tccagagaag
     2041 tacaaggaaa tcttcttcga tcagtctaag aacggatacg ctggatacat cgatggagga
     2101 gcttctcagg aggagttcta caagttcatc aagccaatcc ttgagaagat ggatggaacc
     2161 gaggagcttc ttgttaagtt gaacagagag gatcttctta gaaagcagag aaccttcgat
     2221 aacggaatca tcccacacca gatccacctt ggagagcttc acgctatcct tcgtagacag
     2281 gaggatttct acccattctt gaaggataac agagagaaga tcgagaagat ccttaccttc
     2341 agaatcccat actacgttgg accacttgct agaggaaact ctcgtttcgc ttggatgacc
     2401 agaaagtctg aggagaccat caccccttgg aacttcgaga aggttgttga taagggagct
     2461 tctgctcagt ctttcatcga gagaatgacc aacttcgata agaaccttcc aaacgagaag
     2521 gttcttccaa agcactctct tctttacgag tacttcaccg tttacaacga gcttaccaag
     2581 gttaagtacg ttaccgaggg aatgagaaag ccagctttcc tttctggaga ccagaagaag
     2641 gctatcgttg atcttctttt caagaccaac agaaaggtta ccgttaagca gttgaaggag
     2701 gattacttca agaagatcga gtgcttcgat tctgttgaaa tctctggagt tgaggataga
     2761 ttcaacgctt ctcttggaac ctaccacgat cttttgaaga tcatcaaaga taaggacttc
     2821 cttgataacg aagagaacga ggatatcctc gaggacatcg ttcttacctt gacccttttc
     2881 gaggatagag agatgatcga ggagagactc aagacctacg ctcacctttt cgatgataag
     2941 gttatgaagc agttgaagag aagaagatac accggatggg gtagactttc tcgtaagttg
     3001 atcaacggaa tcagagataa gcagtctgga aagaccatcc ttgatttctt gaagtctgat
     3061 ggattcgcta acagaaactt catccagctt atccacgatg attctcttac cttcaaggag
     3121 gacatccaga aggctcaggt ttctggacag ggagattctc ttcacgagca catcgctaac
     3181 cttgctggat ctccagctat caagaaggga atccttcaga ccgttaaggt tgttgatgag
     3241 cttgttaagg ttatgggtag acacaagcca gagaacatcg ttatcgagat ggctagagag
     3301 aaccagacca cccagaaggg acagaagaac tctcgtgaga gaatgaagag aatcgaggag
     3361 ggaatcaagg agcttggatc tcaaatcttg aaggagcacc cagttgagaa cacccagctt
     3421 cagaacgaga agttgtacct ttactacctt cagaacggaa gagatatgta cgttgatcag
     3481 gagcttgaca tcaacagact ttctgattac gatgttgatc acatcgttcc acagtctttc
     3541 ttgaaggatg attctatcga taacaaggtt cttacccgtt ctgataagaa cagaggaaag
     3601 tctgataacg ttccatctga ggaggttgtt aagaagatga agaactactg gagacagctt
     3661 cttaacgcta agttgatcac ccagagaaag ttcgataacc ttaccaaggc tgagagagga
     3721 ggactttctg agcttgataa ggctggattc atcaagagac agcttgttga gaccagacag
     3781 atcaccaagc acgttgctca gatccttgat tctcgtatga acaccaagta cgatgagaac
     3841 gataagttga tcagagaggt taaggttatc accttgaagt ctaagttggt ttctgatttc
     3901 agaaaggatt tccagttcta caaggttaga gagatcaaca actaccacca cgctcacgat
     3961 gcttacctta acgctgttgt tggaaccgct cttatcaaga agtacccaaa gttggagtct
     4021 gagttcgttt acggagatta caaggtttac gatgttagaa agatgatcgc taagtctgag
     4081 caggagatcg gaaaggctac cgctaagtac ttcttctact ctaacatcat gaacttcttc
     4141 aagaccgaga tcacccttgc taacggagag atcagaaaga gaccacttat cgagaccaac
     4201 ggagagaccg gagagatcgt ttgggataag ggaagagatt tcgctaccgt tagaaaggtt
     4261 ctttctatgc cacaggttaa catcgttaag aaaaccgagg ttcagaccgg aggattctct
     4321 aaggagtcta tccttccaaa gagaaactct gataagttga tcgctagaaa gaaggattgg
     4381 gacccaaaga agtacggagg attcgattct ccaaccgttg cttactctgt tcttgttgtt
     4441 gctaaggttg agaagggaaa gtctaagaag ttgaagtctg ttaaggagct tcttggaatc
     4501 accatcatgg agcgttcttc tttcgagaag aacccaatcg atttccttga ggctaaggga
     4561 tacaaggagg ttaagaagga tcttatcatc aagttgccaa agtactctct tttcgagctt
     4621 gagaacggaa gaaagagaat gcttgcttct gctggagtgc ttcagaaggg aaacgagctt
     4681 gctcttccat ctaagtacgt taacttcctt taccttgctt ctcactacga gaagttgaag
     4741 ggatctccag aggataacga gcagaagcag cttttcgttg agcagcacaa gcactacctt
     4801 gatgagatca tcgagcaaat ctctgagttc tctaagagag ttatccttgc tgatgctaac
     4861 cttgataagg ttctttctgc ttacaacaag cacagagata agccaatcag agagcaggct
     4921 gagaacatca tccacctttt cacccttacc aaccttggtg ctccagctgc tttcaagtac
     4981 ttcgatacca ccatcgatag aaaaagatac acctctacca aggaggttct tgatgctacc
     5041 cttatccacc agtctatcac cggactttac gagaccagaa tcgatctttc tcagcttgga
     5101 ggagataaga gaccagctgc taccaagaag gctggacagg ctaagaagaa gaagggagac
     5161 gacggaggtg gcggtacagg agggggtggg tccgctgagt atgtcagggc gttgttcgac
     5221 ttcaatggaa acgacgagga agatctgcct tttaaaaagg gagatattct caggatcaga
     5281 gataagccgg aagaacaatg gtggaacgct gaagactctg aaggtaagag aggtatgatt
     5341 cttgtcccct acgtcgagaa gtattcgggt gactataaag accacgatgg agattataag
     5401 gaccacgata tagattataa ggatgatgat gataagagcg gaatgaccga tgcagagtac
     5461 gtcaggattc atgagaaact tgacatctac acgtttaaga aacagttttt caacaacaaa
     5521 aaatctgtta gtcaccgctg ttacgtgctg ttcgaattga aacgcagagg tgagaggaga
     5581 gcctgctttt ggggctatgc cgtcaacaag ccgcaaagcg gcacagaaag gggcattcac
     5641 gcggagatat ttagcattag aaaggtcgag gaataccttc gggataatcc cgggcaattc
     5701 actatcaatt ggtactcttc atggtccccg tgtgcagatt gcgctgaaaa gatactggag
     5761 tggtataatc aagaactcag aggaaacggt cacaccctca agatttgggc ttgcaagctt
     5821 tactacgaga aaaatgcaag gaaccagatc ggcctctgga acttgcgcga caacggcgtg
     5881 gggttgaatg tgatggtctc ggagcattac cagtgctgcc ggaagatatt cattcagtcg
     5941 tcacataatc aattgaacga gaataggtgg ctcgaaaaaa ccctgaagcg ggccgagaag
     6001 tggaggagtg aactctcgat aatgatccag gttaaaatac tgcatactac caaatctccg
     6061 gcggtgggac cgaagaagaa gcgcaaggtg gggaccatga ctaatctctc agatataatc
     6121 gagaaggaaa caggaaagca actggtcatc caagaatcga ttttgatgct tcccgaagaa
     6181 gtcgaagaag ttataggaaa taagcccgag tctgacatac tggttcacac agcgtacgat
     6241 gaaagtacgg acgagaatgt catgttgctg acatcggacg cacctgaata caagccttgg
     6301 gctctggtca tacaagatag taacggagaa aataagatta aaatgcttga aggaaggggc
     6361 tccctgctga catgtggaga tgtggaagag aatccaggac catccaggat gatagagcaa
     6421 gatggttgta cccaagtgct gcggcccctc ggatggcggg ggtacagcgc catgactggc
     6481 cataatagac agtccgctgc tctcatgcct ccatgcagcg gatgtcagag gaggggagct
     6541 aggttcttcc tgtctagacc tacctgcccc gtgccctagt ggcggccgcc gacgtccgat
     6601 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     6661 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     6721 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     6781 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     6841 ttactagatc gggaattgat cccccctcga cagcttccgg aaagggcgaa ttcgcaactt
     6901 tgtatacaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     6961 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatgcc
     7021 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     7081 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     7141 gcctcctctg gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     7201 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     7261 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     7321 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     7381 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     7441 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     7501 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     7561 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     7621 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     7681 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     7741 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     7801 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     7861 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     7921 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7981 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     8041 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     8101 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     8161 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     8221 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     8281 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     8341 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8821 aca
//
