LOCUS       sequence-245058-        8439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pYPQ255-x3.7
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..76
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     310..327
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(486..513)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(605..691)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     740..762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     754..771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     755..771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    787..886
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             902..1588
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1643..1690
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             1697..1717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1742..5842
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5843..5890
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5912..6160
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6173..6193
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6215..6467
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6512..6635
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6652..6671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6653..6671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6676..6692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6676..6692)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7123..7914
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(8007..8439,1..156)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
       61 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      181 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      241 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      301 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      361 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      421 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcga gccaggaaga gtttgtagaa
      481 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
      541 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
      601 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      661 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
      721 acgcttgcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
      781 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      841 gagcaatgct tttttataat gccaactttg tacaaaaaag caggctccga attcgccctt
      901 catgagttct gaaacaggtc cagtggctgt cgatccgact ctgaggaggc gcatcgaacc
      961 acacgaattt gaggtttttt ttgatccaag agagcttcgc aaggaaactt gtctcctgta
     1021 tgagataaat tggggaggaa gacatagtat ctggagacac acaagccaga acaccaataa
     1081 gcatgtcgaa gttaacttca tcgaaaaatt tacgaccgaa cgctactttt gtcctaatac
     1141 acgctgcagt atcacttggt tcctttcgtg gtcaccttgc ggtgaatgtt ccagagcaat
     1201 tacggagttt ctctcgagat atccacacgt tacacttttc atttacatag ctcggcttta
     1261 tcatcacgca gatccgagaa atcgccaggg gctgcgcgat ctgatttcgt ccggtgtcac
     1321 cattcaaatt atgactgagc aggaatctgg atactgctgg cgcaacttcg tgaactacag
     1381 tccgtcgaat gaagcgcact ggccgagata tccccatctc tgggtgcgct tgtatgtttt
     1441 ggagctttat tgtatcatct tgggattgcc accttgtctt aatatattgc gccggaaaca
     1501 accgcagctc acttttttca ccattgctct gcagtcttgt cactaccaac gcctcccccc
     1561 gcacatcctg tgggccacag gccttaaatc aggttctgag acccctggca caagtgagtc
     1621 agcaacaccc gagtccacca tggattacaa ggatgatgat gataaggatt acaaggatga
     1681 tgatgataag atggctccaa agaagaagag aaaggttgga atccacggag ttccagctgc
     1741 tgataagaag tactctatcg gacttgccat cggaaccaac tctgttggat gggctgttat
     1801 caccgatgag tacaaggttc catctaagaa gttcaaggtt cttggaaaca ccgatagaca
     1861 ctctatcaag aagaacctta tcggtgctct tcttttcgat tctggagaga ccgctgaggc
     1921 taccagattg aagagaaccg ctagaagaag atacaccaga agaaagaaca gaatctgcta
     1981 ccttcaggaa atcttctcta acgagatggc taaggttgat gattctttct tccacagact
     2041 tgaggagtct ttccttgttg aggaggataa gaagcacgag agacacccaa tcttcggaaa
     2101 catcgttgat gaggttgctt accacgagaa gtacccaacc atctaccacc ttagaaagaa
     2161 gttggttgat tctaccgata aggctgatct tagacttatc taccttgctc ttgctcacat
     2221 gatcaagttc agaggacact tccttatcga gggagacctt aacccagata actctgatgt
     2281 tgataagttg ttcatccagc ttgttcagac ctacaaccag cttttcgagg agaacccaat
     2341 caacgcttct ggagttgatg ctaaggctat cctttctgct agactttcta agtctcgtag
     2401 acttgagaac cttatcgctc agcttccagg agagaagaag aacggacttt tcggaaacct
     2461 tatcgctctt tctcttggac ttaccccaaa cttcaagtct aacttcgatc ttgctgagga
     2521 taccaagttg cagctttcta aggataccta cgatgatgat cttgataacc ttcttgctca
     2581 gatcggagat cagtacgctg atcttttcct tgctgctaag aacctttctg atgctatcct
     2641 tctttctgac atccttagag ttaacaccga gatcaccaag gctccacttt ctgcttctat
     2701 gatcaagctc tacgatgagc accaccagga tcttaccctt ttgaaggctc ttgttagaca
     2761 gcagcttcca gagaagtaca aggaaatctt cttcgatcag tctaagaacg gatacgctgg
     2821 atacatcgat ggaggagctt ctcaggagga gttctacaag ttcatcaagc caatccttga
     2881 gaagatggat ggaaccgagg agcttcttgt taagttgaac agagaggatc ttcttagaaa
     2941 gcagagaacc ttcgataacg gaatcatccc acaccagatc caccttggag agcttcacgc
     3001 tatccttcgt agacaggagg atttctaccc attcttgaag gataacagag agaagatcga
     3061 gaagatcctt accttcagaa tcccatacta cgttggacca cttgctagag gaaactctcg
     3121 tttcgcttgg atgaccagaa agtctgagga gaccatcacc ccttggaact tcgagaaggt
     3181 tgttgataag ggagcttctg ctcagtcttt catcgagaga atgaccaact tcgataagaa
     3241 ccttccaaac gagaaggttc ttccaaagca ctctcttctt tacgagtact tcaccgttta
     3301 caacgagctt accaaggtta agtacgttac cgagggaatg agaaagccag ctttcctttc
     3361 tggagaccag aagaaggcta tcgttgatct tcttttcaag accaacagaa aggttaccgt
     3421 taagcagttg aaggaggatt acttcaagaa gatcgagtgc ttcgattctg ttgaaatctc
     3481 tggagttgag gatagattca acgcttctct tggaacctac cacgatcttt tgaagatcat
     3541 caaagataag gacttccttg ataacgaaga gaacgaggat atcctcgagg acatcgttct
     3601 taccttgacc cttttcgagg atagagagat gatcgaggag agactcaaga cctacgctca
     3661 ccttttcgat gataaggtta tgaagcagtt gaagagaaga agatacaccg gatggggtag
     3721 actttctcgt aagttgatca acggaatcag agataagcag tctggaaaga ccatccttga
     3781 tttcttgaag tctgatggat tcgctaacag aaacttcatc cagcttatcc acgatgattc
     3841 tcttaccttc aaggaggaca tccagaaggc tcaggtttct ggacagggag attctcttca
     3901 cgagcacatc gctaaccttg ctggatctcc agctatcaag aagggaatcc ttcagaccgt
     3961 taaggttgtt gatgagcttg ttaaggttat gggtagacac aagccagaga acatcgttat
     4021 cgagatggct agagagaacc agaccaccca gaagggacag aagaactctc gtgagagaat
     4081 gaagagaatc gaggagggaa tcaaggagct tggatctcaa atcttgaagg agcacccagt
     4141 tgagaacacc cagcttcaga acgagaagtt gtacctttac taccttcaga acggaagaga
     4201 tatgtacgtt gatcaggagc ttgacatcaa cagactttct gattacgatg ttgatcacat
     4261 cgttccacag tctttcttga aggatgattc tatcgataac aaggttctta cccgttctga
     4321 taagaacaga ggaaagtctg ataacgttcc atctgaggag gttgttaaga agatgaagaa
     4381 ctactggaga cagcttctta acgctaagtt gatcacccag agaaagttcg ataaccttac
     4441 caaggctgag agaggaggac tttctgagct tgataaggct ggattcatca agagacagct
     4501 tgttgagacc agacagatca ccaagcacgt tgctcagatc cttgattctc gtatgaacac
     4561 caagtacgat gagaacgata agttgatcag agaggttaag gttatcacct tgaagtctaa
     4621 gttggtttct gatttcagaa aggatttcca gttctacaag gttagagaga tcaacaacta
     4681 ccaccacgct cacgatgctt accttaacgc tgttgttgga accgctctta tcaagaagta
     4741 cccaaagttg gagtctgagt tcgtttacgg agattacaag gtttacgatg ttagaaagat
     4801 gatcgctaag tctgagcagg agatcggaaa ggctaccgct aagtacttct tctactctaa
     4861 catcatgaac ttcttcaaga ccgagatcac ccttgctaac ggagagatca gaaagagacc
     4921 acttatcgag accaacggag agaccggaga gatcgtttgg gataagggaa gagatttcgc
     4981 taccgttaga aaggttcttt ctatgccaca ggttaacatc gttaagaaaa ccgaggttca
     5041 gaccggagga ttctctaagg agtctatcct tccaaagaga aactctgata agttgatcgc
     5101 tagaaagaag gattgggacc caaagaagta cggaggattc gattctccaa ccgttgctta
     5161 ctctgttctt gttgttgcta aggttgagaa gggaaagtct aagaagttga agtctgttaa
     5221 ggagcttctt ggaatcacca tcatggagcg ttcttctttc gagaagaacc caatcgattt
     5281 ccttgaggct aagggataca aggaggttaa gaaggatctt atcatcaagt tgccaaagta
     5341 ctctcttttc gagcttgaga acggaagaaa gagaatgctt gcttctgctg gagtgcttca
     5401 gaagggaaac gagcttgctc ttccatctaa gtacgttaac ttcctttacc ttgcttctca
     5461 ctacgagaag ttgaagggat ctccagagga taacgagcag aagcagcttt tcgttgagca
     5521 gcacaagcac taccttgatg agatcatcga gcaaatctct gagttctcta agagagttat
     5581 ccttgctgat gctaaccttg ataaggttct ttctgcttac aacaagcaca gagataagcc
     5641 aatcagagag caggctgaga acatcatcca ccttttcacc cttaccaacc ttggtgctcc
     5701 agctgctttc aagtacttcg ataccaccat cgatagaaaa agatacacct ctaccaagga
     5761 ggttcttgat gctaccctta tccaccagtc tatcaccgga ctttacgaga ccagaatcga
     5821 tctttctcag cttggaggag ataagagacc agctgctacc aagaaggctg gacaggctaa
     5881 gaagaagaag ggagacggat caggcgggtc aacaaacctc agtgacatca tagagaagga
     5941 aactggtaag caactggtta ttcaagagag cattctgatg ctccctgagg aagtcgagga
     6001 agttatagga aacaagcctg agagcgatat actcgtgcat acggcgtatg acgagagcac
     6061 ggatgaaaat gtgatgctct tgaccagcga tgcgccagaa tataaaccct gggcactggt
     6121 tattcaggac tctaacgggg aaaataaaat caagatgctc tcaggtggct ccccaaagaa
     6181 gaaacgcaag gtttagtggc ggccgccgac gtccgatcgt tcaaacattt ggcaataaag
     6241 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     6301 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     6361 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6421 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattgatccc
     6481 ccctcgacag cttccggaaa gggcgaattc gcaactttgt atacaaaagt tgaacgagaa
     6541 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     6601 taatactgta aaacacaaca tatccagtca ctatgccatc cagctgatat cccctatagt
     6661 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     6721 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctggac cagccaggac
     6781 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     6841 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     6901 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     6961 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     7021 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     7081 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     7141 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     7201 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     7261 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     7321 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     7381 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     7441 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     7501 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     7561 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     7621 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     7681 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     7741 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     7801 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     7861 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     7921 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     7981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggac
//
