LOCUS       sequence-245061-        9012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pYPQ256
FEATURES             Location/Qualifiers
     source          1..9012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             122..169
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             176..196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(221..1657,1847..4510)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          1658..1846
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             4511..4558
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4775..4840
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5474..5494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5501..5752
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5753..5806
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     terminator      6002..6254
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6299..6422
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6439..6458)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6440..6458)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6463..6479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6463..6479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6910..7701
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7794..8382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8283..8302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8536..8553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8712..8739)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(8831..8917)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8966..8988
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8980..8997
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8981..8997
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
      121 ggattacaag gatgatgatg ataaggatta caaggatgat gatgataaga tggctccaaa
      181 gaagaagaga aaggttggaa tccacggagt tccagctgct gataagaagt actctatcgg
      241 acttgccatc ggaaccaact ctgttggatg ggctgttatc accgatgagt acaaggttcc
      301 atctaagaag ttcaaggttc ttggaaacac cgatagacac tctatcaaga agaaccttat
      361 cggtgctctt cttttcgatt ctggagagac cgctgaggct accagattga agagaaccgc
      421 tagaagaaga tacaccagaa gaaagaacag aatctgctac cttcaggaaa tcttctctaa
      481 cgagatggct aaggttgatg attctttctt ccacagactt gaggagtctt tccttgttga
      541 ggaggataag aagcacgaga gacacccaat cttcggaaac atcgttgatg aggttgctta
      601 ccacgagaag tacccaacca tctaccacct tagaaagaag ttggttgatt ctaccgataa
      661 ggctgatctt agacttatct accttgctct tgctcacatg atcaagttca gaggacactt
      721 ccttatcgag ggagacctta acccagataa ctctgatgtt gataagttgt tcatccagct
      781 tgttcagacc tacaaccagc ttttcgagga gaacccaatc aacgcttctg gagttgatgc
      841 taaggctatc ctttctgcta gactttctaa gtctcgtaga cttgagaacc ttatcgctca
      901 gcttccagga gagaagaaga acggactttt cggaaacctt atcgctcttt ctcttggact
      961 taccccaaac ttcaagtcta acttcgatct tgctgaggat gctaagttgc agctttctaa
     1021 ggatacctac gatgatgatc ttgataacct tcttgctcag atcggagatc agtacgctga
     1081 tcttttcctt gctgctaaga acctttctga tgctatcctt ctttctgaca tccttagagt
     1141 taacaccgag atcaccaagg ctccactttc tgcttctatg atcaagagat acgatgagca
     1201 ccaccaggat cttacccttt tgaaggctct tgttagacag cagcttccag agaagtacaa
     1261 ggaaatcttc ttcgatcagt ctaagaacgg atacgctgga tacatcgatg gaggagcttc
     1321 tcaggaggag ttctacaagt tcatcaagcc aatccttgag aagatggatg gaaccgagga
     1381 gcttcttgtt aagttgaaca gagaggatct tcttagaaag cagagaacct tcgataacgg
     1441 atctatccca caccagatcc accttggaga gcttcacgct atccttcgta gacaggagga
     1501 tttctaccca ttcttgaagg ataacagaga gaagatcgag aagatcctta ccttcagaat
     1561 cccatactac gttggaccac ttgctagagg aaactctcgt ttcgcttgga tgaccagaaa
     1621 gtctgaggag accatcaccc cttggaactt cgaggaggta agtttctgct tctacctttg
     1681 atatatatat aataattatc attaattagt agtaatataa tatttcaaat atttttttca
     1741 aaataaaaga atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt
     1801 aatttataac ttttctaata tatgaccaaa atttgttgat gtgcaggttg ttgataaggg
     1861 agcttctgct cagtctttca tcgagagaat gaccaacttc gataagaacc ttccaaacga
     1921 gaaggttctt ccaaagcact ctcttcttta cgagtacttc accgtttaca acgagcttac
     1981 caaggttaag tacgttaccg agggaatgag aaagccagct ttcctttctg gagagcagaa
     2041 gaaggctatc gttgatcttc ttttcaagac caacagaaag gttaccgtta agcagttgaa
     2101 ggaggattac ttcaagaaga tcgagtgctt cgattctgtt gaaatctctg gagttgagga
     2161 tagattcaac gcttctcttg gaacctacca cgatcttttg aagatcatca aggataagga
     2221 tttccttgat aacgaggaga acgaggacat ccttgaggac atcgttctta cccttaccct
     2281 tttcgaggat agagagatga tcgaggagag actcaagacc tacgctcacc ttttcgatga
     2341 taaggttatg aagcagttga agagaagaag atacaccgga tggggtagac tttctcgtaa
     2401 gttgatcaac ggaatcagag ataagcagtc tggaaagacc atccttgatt tcttgaagtc
     2461 tgatggattc gctaacagaa acttcatgca gcttatccac gatgattctc ttaccttcaa
     2521 ggaggacatc cagaaggctc aggtttctgg acagggagat tctcttcacg agcacatcgc
     2581 taaccttgct ggatctccag ctatcaagaa gggaatcctt cagaccgtta aggttgttga
     2641 tgagcttgtt aaggttatgg gtagacacaa gccagagaac atcgttatcg agatggctag
     2701 agagaaccag accacccaga agggacagaa gaactctcgt gagagaatga agagaatcga
     2761 ggagggaatc aaggagcttg gatctcaaat cttgaaggag cacccagttg agaacaccca
     2821 gcttcagaac gagaagttgt acctttacta ccttcagaac ggaagagata tgtacgttga
     2881 tcaggagctt gacatcaaca gactttctga ttacgatgtt gatcacatcg ttccacagtc
     2941 tttcttgaag gatgattcta tcgataacaa ggttcttacc cgttctgata agaacagagg
     3001 aaagtctgat aacgttccat ctgaggaggt tgttaagaag atgaagaact actggagaca
     3061 gcttcttaac gctaagttga tcacccagag aaagttcgat aaccttacca aggctgagag
     3121 aggaggactt tctgagcttg ataaggctgg attcatcaag agacagcttg ttgagaccag
     3181 acagatcacc aagcacgttg ctcagatcct tgattctcgt atgaacacca agtacgatga
     3241 gaacgataag ttgatcagag aggttaaggt tatcaccttg aagtctaagt tggtttctga
     3301 tttcagaaag gatttccagt tctacaaggt tagagagatc aacaactacc accacgctca
     3361 cgatgcttac cttaacgctg ttgttggaac cgctcttatc aagaagtacc caaagttgga
     3421 gtctgagttc gtttacggag attacaaggt ttacgatgtt agaaagatga tcgctaagtc
     3481 tgagcaggag atcggaaagg ctaccgctaa gtacttcttc tactctaaca tcatgaactt
     3541 cttcaagacc gagatcaccc ttgctaacgg agagatcaga aagagaccac ttatcgagac
     3601 caacggagag accggagaga tcgtttggga taagggaaga gatttcgcta ccgttagaaa
     3661 ggttctttct atgccacagg ttaacatcgt taagaaaacc gaggttcaga ccggaggatt
     3721 ctctaaggag tctatccttc caaagagaaa ctctgataag ttgatcgcta gaaagaagga
     3781 ttgggaccca aagaagtacg gaggattcga ttctccaacc gttgcttact ctgttcttgt
     3841 tgttgctaag gttgagaagg gaaagtctaa gaagttgaag tctgttaagg agcttcttgg
     3901 aatcaccatc atggagcgtt cttctttcga gaagaaccca atcgatttcc ttgaggctaa
     3961 gggatacaag gaggttaaga aggatcttat catcaagttg ccaaagtact ctcttttcga
     4021 gcttgagaac ggaagaaaga gaatgcttgc ttctgctgga gagcttcaga agggaaacga
     4081 gcttgctctt ccatctaagt acgttaactt cctttacctt gcttctcact acgagaagtt
     4141 gaagggatct ccagaggata acgagcagaa gcagcttttc gttgagcagc acaagcacta
     4201 ccttgatgag atcatcgagc aaatctctga gttctctaag agagttatcc ttgctgatgc
     4261 taaccttgat aaggttcttt ctgcttacaa caagcacaga gataagccaa tcagagagca
     4321 ggctgagaac atcatccacc ttttcaccct taccaacctt ggtgctccag ctgctttcaa
     4381 gtacttcgat accaccatcg atagaaaaag atacacctct accaaggagg ttcttgatgc
     4441 tacccttatc caccagtcta tcaccggact ttacgagacc agaatcgatc tttctcagct
     4501 tggaggagat aagagaccag ctgctaccaa gaaggctgga caggctaaga agaagaaggg
     4561 agacgacgga ggtggcggta caggaggggg tgggtccgct gagtatgtca gggcgttgtt
     4621 cgacttcaat ggaaacgacg aggaagatct gccttttaaa aagggagata ttctcaggat
     4681 cagagataag ccggaagaac aatggtggaa cgctgaagac tctgaaggta agagaggtat
     4741 gattcttgtc ccctacgtcg agaagtattc gggtgactat aaagaccacg atggagatta
     4801 taaggaccac gatatagatt ataaggatga tgatgataag agcggaatga ccgatgcaga
     4861 gtacgtcagg attcatgaga aacttgacat ctacacgttt aagaaacagt ttttcaacaa
     4921 caaaaaatct gttagtcacc gctgttacgt gctgttcgaa ttgaaacgca gaggtgagag
     4981 gagagcctgc ttttggggct atgccgtcaa caagccgcaa agcggcacag aaaggggcat
     5041 tcacgcggag atatttagca ttagaaaggt cgaggaatac cttcgggata atcccgggca
     5101 attcactatc aattggtact cttcatggtc cccgtgtgca gattgcgctg aaaagatact
     5161 ggagtggtat aatcaagaac tcagaggaaa cggtcacacc ctcaagattt gggcttgcaa
     5221 gctttactac gagaaaaatg caaggaacca gatcggcctc tggaacttgc gcgacaacgg
     5281 cgtggggttg aatgtgatgg tctcggagca ttaccagtgc tgccggaaga tattcattca
     5341 gtcgtcacat aatcaattga acgagaatag gtggctcgaa aaaaccctga agcgggccga
     5401 gaagtggagg agtgaactct cgataatgat ccaggttaaa atactgcata ctaccaaatc
     5461 tccggcggtg ggaccgaaga agaagcgcaa ggtggggacc atgactaatc tctcagatat
     5521 aatcgagaag gaaacaggaa agcaactggt catccaagaa tcgattttga tgcttcccga
     5581 agaagtcgaa gaagttatag gaaataagcc cgagtctgac atactggttc acacagcgta
     5641 cgatgaaagt acggacgaga atgtcatgtt gctgacatcg gacgcacctg aatacaagcc
     5701 ttgggctctg gtcatacaag atagtaacgg agaaaataag attaaaatgc ttgaaggaag
     5761 gggctccctg ctgacatgtg gagatgtgga agagaatcca ggaccatcca ggatgataga
     5821 gcaagatggt tgtacccaag tgctgcggcc cctcggatgg cgggggtaca gcgccatgac
     5881 tggccataat agacagtccg ctgctctcat gcctccatgc agcggatgtc agaggagggg
     5941 agctaggttc ttcctgtcta gacctacctg ccccgtgccc tagtggcggc cgccgacgtc
     6001 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     6061 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     6121 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     6181 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     6241 tatgttacta gatcgggaat tgatcccccc tcgacagctt ccggaaaggg cgaattcgca
     6301 actttgtata caaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
     6361 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
     6421 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     6481 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     6541 tcatgcctcc tctggaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     6601 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     6661 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     6721 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     6781 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     6841 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     6901 ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     6961 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     7021 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     7081 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     7141 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     7201 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     7261 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     7321 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     7381 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     7441 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     7501 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     7561 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     7621 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     7681 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt
     7741 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7801 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7861 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7921 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7981 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8041 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8101 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8161 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8221 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8281 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8341 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8401 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8461 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8521 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8581 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8641 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgcgagcca ggaagagttt
     8701 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     8761 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     8821 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     8881 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     8941 gcgttaacgc ttgcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     9001 aagctcgggc cc
//
