LOCUS       sequence-245064-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins encoding Man operon in E. faecalis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Matsumoto Y, Ishii M, Hasegawa S, Sekimizu K
  TITLE     Enterococcus faecalis YM0831 suppresses sucrose-induced 
            hyperglycemia in a silkworm model and in humans.
  JOURNAL   Commun Biol. 2019 May 2;2:157. doi: 10.1038/s42003-019-0407-5. 
            eCollection 2019.
  PUBMED    31069266
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-05-2"; volume: "2:157. doi"; pages: " 
            10.1038/s42003-019-0407-5. eCollection 2019"
COMMENT     Sequence Label: pMan operon
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      5593..6181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7685..8689)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
        1 tgtgctgttt tatcctttac cttgtctaca aaccccttaa aaacgttttt aaaggctttt
       61 aagccgtctg tacgttcctt aaggaattcg agctcggtac ccggggatcc tctgctctta
      121 atatggatat tccagcagaa gaagtttatt ttatcgcaca gttagtcaaa aatgcccagt
      181 gtatcgaaga taaaatcacc gaaattgata cactagatta atacagaaaa atcgatacac
      241 tatttattag tgtatcgatt ttttaatttt cttaaaaaaa cctataattt tctaggtttc
      301 aaaccgatac actaaagttg gcacgaaact tgcttataat aaggtgtccc tttttaccta
      361 aaatttttaa gtaaaaaaac aaaggtccta acctttaaaa atgaatttaa aggagcattc
      421 ccgatggtaa tggatattcg tttagctcga attgatgatc gattaattca tggccaagta
      481 gcaactgttt gggcaaaatt gacgaacatt aatcgcattt tagtggttag tgattccgtt
      541 gctcatgaca atttgagaaa agccttgtta gttcaagctg ctccacctgg tgtcaaagta
      601 aatgtcatca ctgttcaaaa aatgattgat gtttaccctg atcctcgttt tgattcgttt
      661 cgagccatgt tgttattcac taatcctttg gacgtgaaac gagtggtcga aggcggtgta
      721 caactaactt ctgtgaatat tggcggaatg agtttcagca caggcaagcg aatgattacg
      781 aatgcagtcg cagtggatgc caacgattta aaagcatttc tgtttttaaa tgaacaaggt
      841 attgaattgg aaattcgaaa agtcgcagca gacagccaag tgaatttgat ggatttactg
      901 aaaaaagagc gtagcaaaga aaataacgcc agctgacgtt aatttttaca gacgaaagac
      961 attgaaagga tgtggtggcc taggaccaac atcattgctt catgaactgt attgataaag
     1021 gcaagtgctg atgttgaaaa aaatcaagaa ctattgaaat tttttcattt atttttgtca
     1081 gaatctgatt tatctgattt ttttcaaaaa gtgaaagcgt ttagcagtac aaccatgaaa
     1141 aaaaacatca attatacagg aggtataaaa atggtaggaa ttatcctagc aagtcacggt
     1201 gaatttgctg aagggatctt gcaatctggt gcaatgatct ttggcgaaca agaaaatgta
     1261 aaagcagtta cgttaatgcc tagcgaaggt cccgatgatg taaaagcaaa aatgcaagaa
     1321 gccattgcat cgtttgacaa tcaagacgaa gtactatttt tagttgacct atggggagga
     1381 acaccattta accaagcgaa ctcattgtta gaagatcata aagacaaatg ggcaatcgtt
     1441 gcaggtatga acttaccaat ggttattgaa gcatatgctt ctcgtttctc tatggaatca
     1501 gcacaagaaa ttgcgacaca cattcttgaa acagcaaaag atggtgtcaa agttaaacca
     1561 gaagagttac aaccagcaga agcaccaaaa gctgctgcga cagaagatgc acagccaaaa
     1621 ggttcgttac caccaggtac agttgttggc gatggcaaaa ttaaatttgt gctagcacgt
     1681 attgattcac gtttattaca tggtcaagta gcaacagctt ggacaaaagc aacacaacca
     1741 aacagaatta ttgttgtttc tgatgccgtt gcaaaagatg acttacgtaa gaaattaatc
     1801 gaacaagcag ctcctccagg agtaaaagcc aatgttattc caatcagtaa aatgattgaa
     1861 gtagcgaaag atccacgttt tggcaataca aaagcattat tattatttga aaatcctgaa
     1921 gacgtcttaa aagtggttga aggcggcgta gaaatcccag aagtaaacgt tggttcaatg
     1981 gctcactctg ttggaaaagt cgttgtaagc aaagtattat caatgggtca agaagacgtt
     2041 gatacgtttg atgaattaaa agctaaaggc atcaaatttg atgtgcgtaa agtgccaaat
     2101 gattcaaaag cgaatatgga cgaaattttg aaaaaagcaa aaaatgagtt agcaaacgcg
     2161 taactcgcca aatacaaatc aaaataggag gcttatcatg tctgttatat caattatttt
     2221 agttttactc gttgcctttc tagctggtat ggaagggatt ttggatgagt tccaattcca
     2281 ccaaccgtta gtagcatgta cattaatcgg attagttact ggtaatttag aagccggaat
     2341 tgttttaggt ggtactttac aaatgatcgc gttaggttgg gcgaacatcg gggctgccgt
     2401 tgcaccggat gccgctttag catctgttgc ctcagcaatt attttagttt taggtggcca
     2461 aggtgttaaa ggtgttcctt cagcgattgc catcgcagtt cctttagcgg ttgcaggttt
     2521 gttcttaaca atgatcgttc ggacagttgc ggttccaatc gttcacatga tggatgcggc
     2581 tgctgaaaaa ggcaacatta aacaagttga aatgttacac attttcgctg tatgtttaca
     2641 aggtattcgt attgcgattc cagcaggtgc tttactattc attcctgctg acactgttcg
     2701 taatttctta gaatcaatgc cagcttggtt aactgatggt atggctatcg gtggtggtat
     2761 ggtcgttgcc gtaggttatg cattagtaat caacatgatg gcaacaaaag aagtatggcc
     2821 attcttcatt atcggtttcg tagtcgcagc gatttctcaa ttaacattaa tcgcattagg
     2881 tgctttaggt gtcgctttag ctcttattta cttgaactta tctaaaatgg gtggttcttc
     2941 taactcaaat ggcggaggca actctggcga cccattaggc gatattttaa acgactacta
     3001 attctgaagg aggagcaaag aaaatggcag aaaaaattca attaactaaa aaagatcgtt
     3061 tagctgtagc ttggcgttca acatttatcc aaggttcttg gaactatgaa cgtatgcaaa
     3121 acggcggctg ggcattctca atgattccag caatcaaaaa attatataaa acgaaagaag
     3181 aacgttcagc ggcgttaaaa cgtcacttag aattctttaa tacacaccca tacattgcct
     3241 caccaatcct tggtgtaaca ttagctttgg aagaagaacg tgccaatggc gcaccagttg
     3301 atgacgtagc gattcaaggg gttaaagttg ggatgatggg tcctttagcc ggtgtcggtg
     3361 acccagtttt ctggtttact gttcgtccaa tgttaggcgc tttaggtgct tccttagcaa
     3421 tgggtggtaa catcttaggc ccaatcttat tcttcgttgc ttggaactta attcgttggt
     3481 cattcatgtg gtatacacaa gagtttggtt acaaagcagg ttctaaaatt actgatgacc
     3541 tttcaggtgg tttactacaa gatattacaa aaggtgcttc tattttaggg atgttcgtac
     3601 tggccgcatt ggttcaacgt tgggtatcga ttaagttctt accaattgtt tcacaagtta
     3661 agttagacaa aggtgcttat atcgagtggg ataaacttcc tgctggcggt gaaggaatgc
     3721 ataaagcctt tgaacaagtg aaccaaggct tggcgctttc accaacgaaa gttactactt
     3781 tacaagataa cctagatcaa ttgattccag gtctagcacc attactactt acattcttat
     3841 gtatgtggtt attgaagaaa aaagttagcc caatcgttat cattcttggt ttattcgtag
     3901 tcggcgtagt cggccactta atcggcttat tataagaatg atgaggtaag tttattactt
     3961 ctaatcaaaa aaagcggaag agaagtcgag aaacttttct tccgcttttt taaacaaaga
     4021 tgtaattttg tttgtttcga tcaggaaaga aacgtataat aaagaggaat tagtcgaatg
     4081 aaatggagca gataacatgg tacaatcatt aaacacaaaa gttgatttag ttatagatgc
     4141 gacggcattt acaggattaa cagattatgg acaaattatg ataggtgacc gcgggtttga
     4201 attttaccat gccaaagatc cacgtaagtt tatccaaatt ccgtgggaag aagtcgatta
     4261 cgtgttggct tcagtaatgt tcaaaggaaa atggattcct cgttatgcga ttcaaaccaa
     4321 gaaaaatggg acgtatacgt ttgcttcaaa agagccgaag cgagtgttac gagctattcg
     4381 tgaatatgtt gacccagatc atatggtcca atctttgagt ttctttgatg tcataaaaag
     4441 agggatcctc tcgattggga aaaagaaaaa ataatttata actgttactt ttttataaac
     4501 tagatgaaaa agtagcggtt tttttgtggg aaaacgaggg acttatttat acaaaaaagg
     4561 cacaattttg caccaaaaaa gagacaatca agtgatacgt tgtttttaat ggagttatca
     4621 ttgaaatggt tttcagaaag cacgatgaaa atatttgaca ccttgtaaaa tagtagtaac
     4681 ataataagtg taataaatta ttttatagag gagtgagtgt catggaacaa cgacaacatc
     4741 atggaatgga ctggggttca ttagtattag gaattctatt tgttctgact gcattaattt
     4801 ctttccaaaa tcctgccggt aatttaattg ccattgtgat ggtttttgcg atttttgcta
     4861 ttattaaagg gattttcgaa atttttgtac gtaatcgtat gaaagaattg ctaggctaca
     4921 aagcttatgc accaatcatc ttagggatta ttgatatcct aattggtgtt tacttattgt
     4981 ttaacttaaa tattggtgtc gctgtgttac catttgtttt tgcaatttgg tttttattcg
     5041 actcaatttt cggcttattt actttagatt ttgctaaacg agtaagcaca ggttacttct
     5101 ggttcacatt aattgttgac gttttaggga ttatcttagg tgtaatgtta ttatttaatc
     5161 cattgtcttc agcattaact ctaagcttct tagttggctt ctactttatg atgtttggta
     5221 ttagcaatat tgtatatgct tttagataaa agaagtaaag gaaaacctgt tttttttaat
     5281 aacgacggcg gcagttgcca ttatttcccg aaattgttcg aataagccac acgataatcg
     5341 ctacgggaac taaaatttta gagtcgacct gcaggcatgc aagcttggca ctggcggtgc
     5401 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5461 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5521 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5581 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5641 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5701 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5761 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5821 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5881 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5941 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6001 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6061 gcgcacgagg gagcttccag ggggaaacgc ctggtatcgt tatagtcctg tcgggtttcg
     6121 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6181 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaggc
     6241 aaccacttcc aagtaaagta taacacacta tactttatat tcataaagtg tgtgctctgc
     6301 gaggctgtcg gcagtgccga ccaaaaccat aaaaccttta agacctttct tttttttacg
     6361 agaaaaaaga aacaaaaaaa cctgccctct gccacctcag caaagggggg ttttgctctc
     6421 gtgctcgttt aaaaatcagc aagggacagg tagtattttt tgagaagatc actcaaaaaa
     6481 tctccacctt taaacccttg ccaattttta ttttgtccgt tttgtctagc ttaccgaaag
     6541 ccagactcag caagaataaa atttttattg tctttcggtt ttctagtgta acggacaaaa
     6601 ccactcaaaa taaaaaagat acaagagagg tctctcgtat cttttattca gcaatcggat
     6661 ctggagctgt aatataaaaa ccttcttcaa ctaacggggc aggttagtga cattagaaaa
     6721 ccgactgtaa aaagtacagt cggcattatc tcatattata aaagccagtc attaggccta
     6781 tctgacaatt cctgaataga gttcataaac aatcctgcat gataaccatc acaaacagaa
     6841 tgatgtacct gtaaagatag cggtaaatat attgaattac ctttattaat gaattttcct
     6901 gctgtaataa tgggtagaag gtaattacta ttattattga tatttaagtt aaacccagta
     6961 aatgaagtcc atggaataat agaaagagaa aaagcatttt caggtatagg tgttttggga
     7021 aacaatttcc ccgaaccatt atatttctct acatcagaaa ggtataaatc ataaaactct
     7081 ttgaagtcat tctttacagg agtccaaata ccagagaatg ttttagatac accatcaaaa
     7141 attgtataaa gtggctctaa cttatcccaa taacctaact ctccgtcgct attgtaacca
     7201 gttctaaaag ctgtatttga gtttatcacc cttgtcacta agaaaataaa tgcagggtaa
     7261 aatttatatc cttcttgttt tatgtttcgg tataaaacac taatatcaat ttctgtggtt
     7321 atactaaaag tcgtttgttg gttcaaataa tgattaaata tctcttttct cttccaattg
     7381 tctaaatcaa ttttattaaa gttcatttga tatgcctcct aaatttttat ctaaagtgaa
     7441 tttaggaggc ttacttgtct gctttcttca ttagaatcaa tcctttttta aaagtcaatg
     7501 ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca tggcacctgc cattgctacc
     7561 tgtacagtca aggatggtag aaatgttgtc ggtcctatta ataactcgtc ttcctaagca
     7621 tccttcaatc cttttaataa caattatagc atctaatctt caacaaactg gcccgtttgt
     7681 tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca ataatagaaa
     7741 atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct tcttctgtgt
     7801 catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag attaaccttt
     7861 tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct tcatcatcgg
     7921 tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc gattgtatat
     7981 ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga tcatagtcta
     8041 atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag ttttctgtat
     8101 tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta taagaattat
     8161 ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt ttattaattt
     8221 ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc ttatttaatt
     8281 cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga actgttggct
     8341 tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc attgctctcc
     8401 tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac aactttcttt
     8461 cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt actctttcag
     8521 cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg attttctttt
     8581 ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt gatttttcat
     8641 ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc tatttagtta
     8701 tttgtttagt cacttataac tttaacagat ggggtttttc tgtgcaacca attttaaggg
     8761 ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca gcaactaaaa
     8821 taaaaatgac gttatttcta tatgtatcaa gataagaaag aacaagttca aaaccatcaa
     8881 aaaaagacac cttttcaggt gcttttttta ttttataaac tcattccctg atctcgactt
     8941 cgttcttttt ttacctctcg gttatgagtt agttcaaatt cgttcttttt aggttctaaa
     9001 tcgtgttttt cttggaat
//
