LOCUS       sequence-245088-        7402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a mutated version of mouse Fndc3b circular RNA with the 
            miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Garikipati VNS, Verma SK, Cheng Z, Liang D, Truongcao MM, Cimini M, 
            Yue Y, Huang G, Wang C, Benedict C, Tang Y, Mallaredy V, Ibetti J, 
            Grisanti L, Schumacher SM, Gao E, Rajan S, Wilusz JE, Goukassian D, 
            Houser SR, Koch WJ, Kishore R
  TITLE     Circular RNA CircFndc3b modulates cardiac repair after myocardial 
            infarction via FUS/VEGF-A axis.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4317. doi: 10.1038/s41467-019-11777-7.
  PUBMED    31541092
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4317. doi"; pages: " 
            10.1038/s41467-019-11777-7"
COMMENT     Sequence Label: pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs
FEATURES             Location/Qualifiers
     source          1..7402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2993..3010)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2999..3223
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3269..3697
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3356..3375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3566..3587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3706..3726)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3711..4040
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3891..4026
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3953..3972
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4107..4901
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4161..4180)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4771..4790
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5075..5196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5112..5131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5166..5185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5245..5261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5245..5261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5258..5280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5269..5285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5293..5299,5300..5317,5318..5323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5338..5359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5476..5493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5647..6235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5727..5746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6406..7197,7198..7266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7029..7048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7267..7371)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattccat
      961 tgagaaatga ctgagttccg gtgctctcaa gtcattgatc tttgtcgact tttatttggt
     1021 ctctgtaata acgacttcaa aaacattaaa ttctgttgcg aagccagtaa gctacaaaaa
     1081 gaaaaaacaa gagagaatgc tatagtcgta tagtatagtt tcccgactat ctgataccca
     1141 ttacttatct agggggaatg cgaacccaaa attttatcag ttttctcgga tatcgataga
     1201 tattggggaa taaatttaaa taaataaatt ttgggcgggt ttagggcgtg gcaaaaagtt
     1261 ttttggcaaa tcgctagaaa tttacaagac ttataaaatt atgaaaaaat acaacaaaat
     1321 tttaaacacg tgggcgtgac agttttgggc ggttttaggg cgttagagta ggcgaggaca
     1381 gggttacatc gactaggctt tgatcctgat caagaatata tatactttat accgcttcct
     1441 tctacatgtt acctattttt caacgaatct agtatacctt tttactgtac gatttatggg
     1501 tataataata agctaaatcg agactaagtt ttattgttat atatattttt tttattttat
     1561 gcagaaatta attaagtagc aggttgaggg caattctcca tgaatgtacg tcaccatgat
     1621 gatgaccgac cagatccccc tggaattgcc acgagacgtc aatggggagg tggccatgat
     1681 gccccacttg gtgaacggag aagcagctgt ccaggtaaga tttgttcctg ttttattaat
     1741 tgttctcctt tgtttgtcta agatgagtga gcactgaatg gaagttagat gtgtttggca
     1801 ccggtgcatg atacagtata atttccagct tattaattat ggttgtgttt aattattttt
     1861 taatgaataa aaagtattct atggttttaa taaaaacgtt aggttatacc attatgacga
     1921 ccttattgaa ccattatatt gccatgtacc ctttgtataa cactttgagg atacaaccag
     1981 aaaataacca ttagaacttt tctctttttt gacaggtcat tcttgtccaa gtgaaccctc
     2041 cactgacctt taccatcaga gccgaggatg ggacacttca gtgcatccaa gccgcggagc
     2101 gtaagtattc aaaattccaa aattttttac tagaaatatt cgatttttta ataggcagtt
     2161 tctatactat tgtatactat tgtagattcg ttgaaaagta tgtaacagga agaataaagc
     2221 atttccgacc atgtaaagta tatatattct taataaggat caatagccga gtcgatctcg
     2281 ccatgtccgt ctgtcttatt gttttattac cgccgagaca tcaggaacta taaaagctag
     2341 aaggatgagt tttagcatac agattctaga gacaaggacg cagagcaagt ttgttgatcc
     2401 atgctgccac gctttaactt tctcaaattg cccaaaactg ccatgcccac atttttgaac
     2461 tattttcgaa attttttcat aattgtatta ctcgtgtaaa tttccatcaa tttgccaaaa
     2521 aactttttgt cacgcgttaa cgccctaaag ccgccaattt ggtcacgccc acactattga
     2581 gcaattatca aattttttct cattttattc cccaatatct atcgatatcc ccgattatga
     2641 aattattaaa tttcgcgttc gcattcacac tagctgagta acgagtatct gatagttggg
     2701 gaaatcgact tattttttat atacaatgaa aatgaattta atcatatgaa tatcgattat
     2761 agctttttat ttaatatgaa tatttatttg ggcttaaggt gtaacctcct cgacataaga
     2821 ctcacatggc gcaggcacat tgaagacaaa aatactcatt gtcgggtctc gcaccctcca
     2881 gcagcaccta aaattatgtc ttcaattatt gccaacattg gagacacaat tagtctgtgg
     2941 cacctcaggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     3001 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3061 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3121 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3181 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     3241 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     3301 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3361 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3421 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3481 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3541 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3601 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3661 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     3721 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3781 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3841 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3901 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3961 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4021 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4081 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4141 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4201 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4261 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4321 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4381 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     4441 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4501 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4561 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4621 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4681 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4741 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4801 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4861 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     4921 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4981 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5041 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     5101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5221 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5281 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5341 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5401 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5821 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6061 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6781 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7381 ccgaaaagtg ccacctgacg tc
//
