LOCUS       sequence-24509-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Gupta GP, Perk J, Acharyya S, de Candia P, Mittal V, Todorova-Manova
            K, Gerald WL, Brogi E, Benezra R, Massague J
  TITLE     ID genes mediate tumor reinitiation during breast cancer lung 
            metastasis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Dec 4. 104(49):19506-11.
  PUBMED    18048329
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Dec 4. 104(49):19506-11."
COMMENT     Sequence Label: pRSpuro shID1#6
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     16..32
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(105..319)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(165..184)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        330..829
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(365..388)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     762..781
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cggatgacga tctctcgagg tcgacggtat cgataagctt ttccaaaaag aggaattacg
       61 tgctctgttc tcttgaaaca gagcacgtaa ttcctcgggg atctgtggtc tcatacagaa
      121 cttataagat tcccaaatcc aaagacattt cacgtttatg gtgatttccc agaacacata
      181 gcgacatgca aatattgcag ggcgccactc ccctgtccct cacagccatc ttcctgccag
      241 ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg cccgcgattc cttggagcgg
      301 gttgatgacg tcagcgttcg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
      361 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
      421 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
      481 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
      541 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
      601 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
      661 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
      721 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
      781 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
      841 aagctagctt accatgaccg agtacaagcc ccacggtgcg cctcgccacc cgcgacgacg
      901 tccccanggn cgtaagcacc ctcgccggcc gcgttcgcna ntaccccgcc angcgcaaaa
      961 cgtnnancgn naaccncccn aatttnaggg ggtcacnann nnnaaaaaaa ntnttnnnct
     1021 naacnnngnn gggnttttna atnnnnnaan gggngnnntc gganaaaaaa aanncnc
//
