LOCUS       sequence-245090-        9979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ""sender"" trans-cellular NanoLuc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Kim CK, Cho KF, Kim MW, Ting AY
  TITLE     Luciferase-LOV BRET enables versatile and specific transcriptional 
            readout of cellular protein-protein interactions.
  JOURNAL   eLife. 2019 Apr 3;8. pii: 43826. doi: 10.7554/eLife.43826.
  PUBMED    30942168
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-04-3"; volume: "8. pii: 43826. doi"; pages: " 
            10.7554/eLife.43826"
COMMENT     Sequence Label: pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
FEATURES             Location/Qualifiers
     source          1..9979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1847..1964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2081..2384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2385..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2539..2559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2611..2620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2668..3177
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             5467..5493
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5494..5520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5836..6424
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5889..5909)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6307..6318)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6496..6729
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6807..6928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6844..6863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6898..6917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6968..7103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7123..7142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7124..7142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7152..7169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7152..7168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7161..7183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7310..7738
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7397..7416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7607..7628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7791..7895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7896..7964,7965..8756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8114..8133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8927..9515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9416..9435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9669..9686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9803..9824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9839..9844,9845..9862,9863..9869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9877..9893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9882..9904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9901..9917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9901..9917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9936..9956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9938..9956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc gccaccatgg gagtcaaagt tctgtttgcc
     2641 ctgatctgca tcgctgtggc cgaggccgtc ttcacactcg aagatttcgt tggggactgg
     2701 cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg
     2761 tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat
     2821 gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg
     2881 ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg
     2941 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
     3001 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
     3061 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
     3121 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgggc
     3181 gacccactag tccagtgtgg tggaattgcc ggatccgcca cccagacatc tgtgtccccc
     3241 tcaaaagtca tcctgccccg gggaggctcc gtgctggtga catgcagcac ctcctgtgac
     3301 cagcccaagt tgttgggcat agagaccccg ttgcctaaaa aggagttgct cctgcctggg
     3361 aacaaccgga aggtgtatga actgagcaat gtgcaagaag atagccaacc aatgtgctat
     3421 tcaaactgcc ctgatgggca gtcaacagct aaaaccttcc tcaccgtgta ctggactcca
     3481 gaacgggtgg aactggcacc cctcccctct tggcagccag tgggcaagaa ccttacccta
     3541 cgctgccagg tggagggtgg ggcaccccgg gccaacctca ccgtggtgct gctccgtggg
     3601 gagaaggagc tgaaacggga gccagctgtg ggggagcccg ctgaggtcac gaccacggtg
     3661 ctggtgagga gagatcacca tggagccaat ttctcgtgcc gcactgaact ggacctgcgg
     3721 ccccaagggc tggagctgtt tgagaacacc tcggccccct accagctcca gacctttgtc
     3781 ctgccagcga ctcccccaca acttgtcagc ccccgggtcc tagaggtgga cacgcagggg
     3841 accgtggtct gttccctgga cgggctgttc ccagtctcgg aggcccaggt ccacctggca
     3901 ctgggggacc agaggttgaa ccccacagtc acctatggca acgactcctt ctcggccaag
     3961 gcctcagtca gtgtgaccgc agaggacgag ggcacccagc ggctgacgtg tgcagtaata
     4021 ctggggaacc agagccagga gacactgcag acagtgacca tctacagctt tccggcgccc
     4081 aacgtgattc tgacgaagcc agaggtctca gaagggaccg aggtgacagt gaagtgtgag
     4141 gcccacccta gagccaaggt gacgctgaat ggggttccag cccagccact gggcccgagg
     4201 gcccagctcc tgctgaaggc caccccagag gacaacgggc gcagcttctc ctgctctgca
     4261 accctggagg tggccggcca gcttatacac aagaaccaga cccgggagct tcgtgtcctg
     4321 tatggccccc gactggacga gagggattgt ccgggaaact ggacgtggcc agaaaattcc
     4381 cagcagactc caatgtgcca ggcttggggg aacccattgc ccgagctcaa gtgtctaaag
     4441 gatggcactt tcccactgcc catcggggaa tcagtgactg tcactcgaga tcttgagggc
     4501 acctacctct gtcgggccag gagcactcaa ggggaggtca cccgcaaggt gaccgtgaat
     4561 gtgctctccc cccggtatga gtcttctaat gtagcttcct cctcctccac ctcttcctcg
     4621 ccggggtctc actctcagca cgagcaccat ttccatggca gcaagcatca ctcagtgcct
     4681 atttctatct atcgttcccc tgtttccctt cgaggaggac acgctggcgc tacgtacatc
     4741 tttgggaaaa gtggtgggct tatcctctac acctggccag ccaatgacag gcccagcacg
     4801 cggtctgacc gccttgccgt gggcttcagc accactgtga aggatggcat cttggtccgc
     4861 atcgacagtg ctccaggact tggtgccttc ctccagcttc acatagaaca ggggaaaatt
     4921 ggagttgtct tcaacattgg cacagttgac atctccatca aagaggagag aacccctgta
     4981 aatgacggca aataccatgt ggtacgcttc accaggaacg gcggcaacgc caccctgcag
     5041 gtggacaact ggccagtgaa tgaacattat cctacaggcc ggcagttaac catcttcaac
     5101 actcaggcgc aaatagccat tggtggaaag gacaaaggac gcctcttcca aggccaactc
     5161 tctgggctct attatgatgg tttgaaagta ctgaacatgg cggctgagaa caaccccaat
     5221 attaaaatca atggaagtgt tcggctggtt ggagaagtcc catcaatttt gggaacaaca
     5281 cagacgacct ccatgccacc agaaatgtct actactgtca tggaaaccac tactacaatg
     5341 gcgactacca caacccgtaa gaatcgctct acagccagca ttcagccaac atcagatgat
     5401 cttgtttcat ctgctgaatg ttcaagtgat gatgaagact ttgttgaatg tgagccgagt
     5461 acagcatacc catacgatgt tccagattac gcttatcctt acgacgtacc tgactatgct
     5521 aaccccacgg agccgggaat cagacgggtt ccgggggcct cagaggtgat ccgggagtcg
     5581 agcagcacaa cagggatggt cgtcggcatt gtggctgctg ccgccctctg catcttgatc
     5641 ctcctgtacg ccatgtacaa gtacaggaac agggacgagg ggtcctatca agtggacgag
     5701 acgcggaact acatcagcaa ctccgcccag agcaacggca cgctcatgaa ggagaagcag
     5761 cagagctcga agagcggcca caagaaacag aaaaacaagg acagggagta ttacgtgtag
     5821 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5881 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5941 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6001 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6061 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6121 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6181 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6241 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6301 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6361 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6421 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6481 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6541 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6601 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6661 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6721 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6781 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6901 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7081 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7141 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7201 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7261 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7501 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7621 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7681 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7741 aacaaaatat taaygcttas amktyaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7861 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7921 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7981 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8041 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8101 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     8161 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8221 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8281 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8341 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8401 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     8461 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8521 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8581 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8641 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8701 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8761 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8821 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9061 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ccyratrcgs wawyykcyyc
     9721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9901 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     9961 aaaagctgga gctgcaagc
//
