LOCUS       sequence-245103-        2940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_APikL2
FEATURES             Location/Qualifiers
     source          1..2940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(764..780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(764..780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(777..799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    788..804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(812..818,819..836,837..842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    857..878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(995..1012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1166..1754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1246..1265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1925..2740)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1925..2740)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2648..2667
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2741..2845)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2913..2931
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgcaaaatg aatatattga tgctaaaaaa catggtattg atttgtctag
      481 agaaagagct ccaaattttg ttgatcatcc aggtattcca ccatctgatt gtttttggtt
      541 tttgtataaa aattatgtta gacaaaatgc tggtgtttgt caatctgatt ggtcttttga
      601 tatgaaaatt ggtcaatatt gggttactat tcatactgat gaaggttgta gattgtctgg
      661 tattattcca gctggttggt tgattttggg tatgaaaaga ccaggttttt gagcttagag
      721 accaagttca tcccaatggc gcgccgagct tggctcgagc atggtcatag ctgtttcctg
      781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      901 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      961 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1141 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1201 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1261 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1321 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1381 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1441 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1501 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1561 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1621 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1681 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1741 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1801 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1861 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1921 acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     1981 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     2041 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     2101 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     2161 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     2221 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     2281 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     2341 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     2401 gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg
     2461 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     2521 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     2581 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     2641 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     2701 gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt
     2761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     2881 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
