LOCUS       sequence-245105-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QUAS::CFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC812
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2235..2257)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2357..2376
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2454..2472)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2540..2644
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2645..2713,2714..3505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2863..2882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3676..4264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4165..4184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4418..4435
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4552..4573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta
      121 actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat
      181 ggatacgcta acaacttgga aatgaaataa gcttgcatgc ccaattcgga tccgggtaat
      241 cgcttatcct cggataaaca attatcctca cgggtaatcg cttatccgct cgggtaatcg
      301 cttatcctcg ggtaatcgct tatccttaag cttgatatct ctaggatcga ttttttgcaa
      361 attacgagcg ttgtaggggg cggagcgata ggtcctatag gttttggtat atcatcattc
      421 attcattcat tggtacattc atttacccac cttcctcttt ctgagcttct ctggagttct
      481 gtgcttcctt tttcccttat ctttatactg taatttttaa ctttcagccg gtagaaaaaa
      541 tggtttctaa gggagaagaa cttttcactg gagttgttcc aattcttgtt gaattggatg
      601 gagatgttaa cggacataag ttctcagttt ctggagaagg agaaggagat gctacatacg
      661 gaaaacttac tttgaagttc atctgtacaa ctggaaaatt gccagttcca tgggtaagtt
      721 taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca cttgttacaa
      781 ctttgacttg gggagttcaa tgctttgcaa gatacccaga tcatatgaag caacatgatt
      841 tctttaagtc agctatgcca gaaggatacg ttcaagaaag aacaattttc tttaaggatg
      901 atggaaacta caagacacga gcagaggtta agttcgaagg agatactctt gttaacagaa
      961 tcgaattgaa gggaatcgat tttaaagaag atggaaacat ccttggacat aagttggaat
     1021 acaatgctat ctctgataac gtttacatca ctgcagataa gcaaaagaat ggaattaaag
     1081 ctaacttcaa gattcgacat aatattgaag atggatcagt tcaacttgct gatcattacc
     1141 aacaaaacac accaattgga gatggaccag ttcttttgcc agataaccat tacctttcaa
     1201 ctcaatctaa gttgtcaaag gatccaaacg aaaagagaga tcatatggtt cttttggaat
     1261 tcgttacagc tgcaggaatt actcttggaa tggatgaatt gtataaataa gcattcgtag
     1321 aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg
     1381 ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa
     1441 ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     1501 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     1561 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     1621 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     1681 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     1741 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     1801 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     1861 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     1921 agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag
     1981 atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     2041 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     2101 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     2161 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     2221 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2281 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2341 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     2401 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt
     2461 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2521 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2581 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2641 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2701 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2761 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2821 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2881 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2941 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3001 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3061 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3121 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3181 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3241 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3301 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3361 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3421 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3481 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3541 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3601 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3661 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3721 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3781 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3841 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3901 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3961 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4021 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4081 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4141 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4201 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4261 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4321 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4381 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4441 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4501 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4561 ttagctcact cattaggcac cccaggc
//
